奇异变形杆菌和普通变形杆菌菌株的一种新型噬菌体分型方案。3. 裂解特性分析。

A new bacteriophage typing scheme for Proteus mirabilis and Proteus vulgaris strains. 3. Analysis of lytic properties.

作者信息

Sekaninová G, Rychlík I, Kolárová M, Pillich J, Seménka J, Zajícová V

机构信息

Department of Epidemiology of Infectious Diseases, Faculty of Medicine, Masaryk University, Brno, Czech Republic.

出版信息

Folia Microbiol (Praha). 1998;43(2):136-40. doi: 10.1007/BF02816498.

DOI:10.1007/BF02816498

PMID:9721605

Abstract

The lytic properties of 21 bacteriophages constituting a new typing set for Proteus were examined in 507 Proteus mirabilis and 29 P. vulgaris strains isolated from patients and healthy subjects. Comparison of their morphological, serological, genetic and lytic properties showed that, in the Myoviridae and Podoviridae families, some phages were so closely related that the presence of all of them in the set was redundant. Analysis of the lytic properties revealed that some of the bacteriophages were not active enough to facilitate the differentiation of Proteus strains. The size of the final typing set was reduced from 21 to 12 phages but it was suggested that, in order to improve the differentiation capacity of the set, new phages should be included.

摘要

在从患者和健康受试者中分离出的507株奇异变形杆菌和29株普通变形杆菌菌株中，检测了构成变形杆菌新分型组的21种噬菌体的裂解特性。对它们的形态、血清学、遗传学和裂解特性进行比较后发现，在肌尾噬菌体科和短尾噬菌体科中，一些噬菌体之间的关系非常密切，以至于分型组中同时存在所有这些噬菌体显得多余。对裂解特性的分析表明，一些噬菌体的活性不足以促进变形杆菌菌株的鉴别。最终分型组的噬菌体数量从21种减少到12种，但有人建议，为了提高该分型组的鉴别能力，应纳入新的噬菌体。