由噬菌体phiX174诱导的DNA甲基化酶
DNA methylase induced by bacteriophage phiX174.
作者信息
Razin A
出版信息
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3773-5. doi: 10.1073/pnas.70.12.3773.
Abstract
A cytosine-specific DNA methylase activity, which is normally absent in the Escherichia coli B strain, was found to be induced in these cells by infection with bacteriophage varphiX174. In vivo experiments revealed a single 5-methylcytosine residue in the phage DNA molecule and 5-methylcytosine residues in the infected host DNA, in addition to the 6-methylaminopurine residues present in the uninfected cells. In vitro, a partially purified enzyme from infected cells methylated DNA from uninfected cells, but showed no activity with cellular DNA from infected cells. The partially purified methylase derived from uninfected cells lacks this activity.
摘要
一种胞嘧啶特异性DNA甲基化酶活性，通常在大肠杆菌B菌株中不存在，被发现可通过噬菌体φX174感染在这些细胞中诱导产生。体内实验揭示，除了未感染细胞中存在的6-甲基氨基嘌呤残基外，噬菌体DNA分子中存在单个5-甲基胞嘧啶残基，且感染宿主DNA中也存在5-甲基胞嘧啶残基。在体外，来自感染细胞的部分纯化酶可使未感染细胞的DNA甲基化，但对感染细胞的细胞DNA无活性。来自未感染细胞的部分纯化甲基化酶缺乏这种活性。
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