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DNA甲基化的生物学作用研究。II. φX174 DNA甲基化在病毒子代DNA合成过程中的作用。

Studies on the biological role of DNA methylation. II. Role of phiX174 DNA methylation in the process of viral progeny DNA synthesis.

作者信息

Friedman J, Razin A

出版信息

Nucleic Acids Res. 1976 Oct;3(10):2665-75. doi: 10.1093/nar/3.10.2665.

DOI:10.1093/nar/3.10.2665
PMID:136644
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC343120/
Abstract

In vivo inhibition of bacteriophage phiX174 DNA methylation by nicotinamide resulted in the accumulation of replicative intermediates with multiple-genome length single-stranded "tails". These abnormal replicative intermediates could not be chased into viral single-stranded circular DNA. The effect of nicotinamide on phage maturation and accumulation of abnormal replicative intermediates could be reversed by washing out the inhibitor. The results suggest that the single methyl group present in the viral DNA serves as a recognition site for a specific endonuclease, probably the gene A protein product, that is responsible for the excision of the single-stranded one-genome long viral DNA, before final maturation of the virus occurs.

摘要

烟酰胺在体内对噬菌体phiX174 DNA甲基化的抑制作用导致了具有多基因组长度单链“尾巴”的复制中间体的积累。这些异常的复制中间体无法转化为病毒单链环状DNA。通过洗脱抑制剂，烟酰胺对噬菌体成熟和异常复制中间体积累的影响可以逆转。结果表明，病毒DNA中存在的单个甲基作为一种特定核酸内切酶的识别位点，可能是基因A蛋白产物，在病毒最终成熟之前，该酶负责切除单链单基因组长度的病毒DNA。

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Studies on the biological role of DNA methylation. II. Role of phiX174 DNA methylation in the process of viral progeny DNA synthesis.DNA甲基化的生物学作用研究。II. φX174 DNA甲基化在病毒子代DNA合成过程中的作用。
Nucleic Acids Res. 1976 Oct;3(10):2665-75. doi: 10.1093/nar/3.10.2665.
2
Studies on the biological role of DNA methylation: III Role in excision of one-genome long single-stranded phi X 174 DNA.DNA甲基化的生物学作用研究：III. 在单基因组长度的单链φX 174 DNA切除中的作用
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Synthesis of phiX174 viral DNA in vitro depends on phiX replicative form DNA.在体外合成φX174病毒DNA依赖于φX复制型DNA。
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Studies on the biological role of DNA methylation: inhibition of methylation and maturation of the bacteriophage phichi174 by nicotinamide.DNA甲基化的生物学作用研究：烟酰胺对噬菌体φX174甲基化和成熟的抑制作用
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引用本文的文献

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Detection of 5-methylcytosine in DNA sequences.DNA序列中5-甲基胞嘧啶的检测。
Nucleic Acids Res. 1978 May;5(5):1479-85. doi: 10.1093/nar/5.5.1479.
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Cleavage specificity of the restriction endonuclease isolated from Haemophilus gallinarum (Hga I).从鸡嗜血杆菌中分离出的限制性内切酶（Hga I）的切割特异性。
Proc Natl Acad Sci U S A. 1977 Aug;74(8):3213-6. doi: 10.1073/pnas.74.8.3213.
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Distribution of 5-methylcytosine in chromatin.染色质中5-甲基胞嘧啶的分布
Proc Natl Acad Sci U S A. 1977 Jul;74(7):2725-8. doi: 10.1073/pnas.74.7.2725.
4
Studies on the biological role of DNA methylation: III Role in excision of one-genome long single-stranded phi X 174 DNA.DNA甲基化的生物学作用研究：III. 在单基因组长度的单链φX 174 DNA切除中的作用
Nucleic Acids Res. 1977 Oct;4(10):3483-96. doi: 10.1093/nar/4.10.3483.

本文引用的文献

1
Structure of the DNA of bacteriophage phiX174. VII. Methylation.噬菌体φX174的DNA结构。VII. 甲基化
J Mol Biol. 1970 Oct 28;53(2):251-9. doi: 10.1016/0022-2836(70)90298-6.
2
The process of infection with Bacteriophage phi-X174. XXIX. In vivo studies on the synthesis of the single-stranded DNA of progeny phi-X174 bacteriophage.噬菌体φ-X174的感染过程。二十九。子代φ-X174噬菌体单链DNA合成的体内研究。
J Mol Biol. 1969 Oct 28;45(2):237-63. doi: 10.1016/0022-2836(69)90103-x.
3
The process of infection with bacteriophage phiX174. XXVI. Transfer of the parental DNA of bacteriophage phiX174 into progeny bacteriophage particles.噬菌体φX174的感染过程。二十六。噬菌体φX174的亲代DNA向子代噬菌体颗粒的转移。
J Mol Biol. 1969 Feb 14;39(3):641-54. doi: 10.1016/0022-2836(69)90150-8.
4
Formation of the parental replicative form DNA of bacteriophage phi-X174 and initial events in its replication.噬菌体φ-X174亲本复制型DNA的形成及其复制的起始事件。
J Mol Biol. 1971 Nov 14;61(3):565-86. doi: 10.1016/0022-2836(71)90065-9.
5
Location of the 5-methylcytosine group on the bacteriophage phi X174 genome.噬菌体φX174基因组上5-甲基胞嘧啶基团的位置。
J Virol. 1974 Oct;14(4):872-7. doi: 10.1128/JVI.14.4.872-877.1974.
6
Isolation and function of the gene A initiator of bacteriophage phi-chi 174, a highly specific DNA endonuclease.噬菌体φX174的基因A起始子的分离及其功能，一种高度特异性的DNA内切酶
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1549-53. doi: 10.1073/pnas.71.4.1549.
7
DNA methylase induced by bacteriophage phiX174.由噬菌体phiX174诱导的DNA甲基化酶
Proc Natl Acad Sci U S A. 1973 Dec;70(12):3773-5. doi: 10.1073/pnas.70.12.3773.
8
In vivo methylation of replicating bacteriophage phi chi174 DNA.复制型噬菌体φX174 DNA的体内甲基化
J Mol Biol. 1973 Aug 15;78(3):417-25. doi: 10.1016/0022-2836(73)90465-8.
9
Filamentous bacterial viruses. VI. Role of fd gene 2 in deoxyribonucleic acid replication.丝状细菌病毒。VI. fd基因2在脱氧核糖核酸复制中的作用。
J Virol. 1972 Sep;10(3):384-91. doi: 10.1128/JVI.10.3.384-391.1972.
10
Cis-limited action of the gene-A product of bacteriophage phiX174 and the essential bacterial site (E. coli-electron microscopy-cis-acting protein-specifically-nicked RF).噬菌体φX174基因A产物的顺式限制作用及必需细菌位点（大肠杆菌-电子显微镜-顺式作用蛋白特异性切口复制型）
Proc Natl Acad Sci U S A. 1972 Feb;69(2):475-9. doi: 10.1073/pnas.69.2.475.