DNA甲基化的生物学作用研究。II. φX174 DNA甲基化在病毒子代DNA合成过程中的作用。
Studies on the biological role of DNA methylation. II. Role of phiX174 DNA methylation in the process of viral progeny DNA synthesis.
作者信息
Friedman J, Razin A
出版信息
Nucleic Acids Res. 1976 Oct;3(10):2665-75. doi: 10.1093/nar/3.10.2665.
Abstract
In vivo inhibition of bacteriophage phiX174 DNA methylation by nicotinamide resulted in the accumulation of replicative intermediates with multiple-genome length single-stranded "tails". These abnormal replicative intermediates could not be chased into viral single-stranded circular DNA. The effect of nicotinamide on phage maturation and accumulation of abnormal replicative intermediates could be reversed by washing out the inhibitor. The results suggest that the single methyl group present in the viral DNA serves as a recognition site for a specific endonuclease, probably the gene A protein product, that is responsible for the excision of the single-stranded one-genome long viral DNA, before final maturation of the virus occurs.
摘要
烟酰胺在体内对噬菌体phiX174 DNA甲基化的抑制作用导致了具有多基因组长度单链“尾巴”的复制中间体的积累。这些异常的复制中间体无法转化为病毒单链环状DNA。通过洗脱抑制剂，烟酰胺对噬菌体成熟和异常复制中间体积累的影响可以逆转。结果表明，病毒DNA中存在的单个甲基作为一种特定核酸内切酶的识别位点，可能是基因A蛋白产物，在病毒最终成熟之前，该酶负责切除单链单基因组长度的病毒DNA。
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本文引用的文献
1
Structure of the DNA of bacteriophage phiX174. VII. Methylation.噬菌体φX174的DNA结构。VII. 甲基化
J Mol Biol. 1970 Oct 28;53(2):251-9. doi: 10.1016/0022-2836(70)90298-6.
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The process of infection with Bacteriophage phi-X174. XXIX. In vivo studies on the synthesis of the single-stranded DNA of progeny phi-X174 bacteriophage.噬菌体φ-X174的感染过程。二十九。子代φ-X174噬菌体单链DNA合成的体内研究。
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The process of infection with bacteriophage phiX174. XXVI. Transfer of the parental DNA of bacteriophage phiX174 into progeny bacteriophage particles.噬菌体φX174的感染过程。二十六。噬菌体φX174的亲代DNA向子代噬菌体颗粒的转移。
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Formation of the parental replicative form DNA of bacteriophage phi-X174 and initial events in its replication.噬菌体φ-X174亲本复制型DNA的形成及其复制的起始事件。
J Mol Biol. 1971 Nov 14;61(3):565-86. doi: 10.1016/0022-2836(71)90065-9.
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Location of the 5-methylcytosine group on the bacteriophage phi X174 genome.噬菌体φX174基因组上5-甲基胞嘧啶基团的位置。
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