大肠杆菌K-12中乳糖操纵子（lac）和脯氨酸（pro）标记的修正图谱位置。
Modified map positions for lac and the pro markers in Escherichia coli K-12.
作者信息
Broda P
出版信息
J Bacteriol. 1974 Feb;117(2):741-6. doi: 10.1128/jb.117.2.741-746.1974.
Abstract
Interrupted mating experiments were performed with Hfr strains H and C and three leu lac purE recipient strains derived from a common parent and carrying, respectively, the proA(-), proB(-), and proC(-) mutations. It was concluded that if leu is placed at 1.5 min and purE at 12 min from thr, the origin on the Taylor-Trotter map, lac is at about 7.5 min and the pro genes are at about 6.0, 6.6, and 8.4 min, respectively. Both conjugational and transductional data suggest that the strain carrying the proB(-) mutation also carries a second mutation close to the proA site which independently confers a Pro(-) phenotype. The times before the onset of transfer of chromosomal deoxyribonucleic acid by both Hfr strains B4 and B8 were approximately 3 min.
摘要
用Hfr菌株H和C以及三个来自同一亲本的亮氨酸（leu）、乳糖（lac）、嘌呤核苷酸E（purE）受体菌株进行了中断杂交实验，这三个受体菌株分别携带proA（-）、proB（-）和proC（-）突变。得出的结论是，如果按照泰勒 - 特罗特图谱中thr起始点计算，leu位于1.5分钟处，purE位于12分钟处，那么lac大约在7.5分钟处，pro基因分别位于大约6.0、6.6和8.4分钟处。结合转移和转导数据表明，携带proB（-）突变的菌株还在靠近proA位点处携带另一个突变，该突变独立导致脯氨酸缺陷型（Pro（-））表型。Hfr菌株B4和B8转移染色体脱氧核糖核酸开始前的时间约为3分钟。
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