大肠杆菌K-12中的粗略图谱距离和高频重组菌转移时间。
Gross map distances and Hfr transfer times in Escherichia coli K-12.
作者信息
Broda P, Collins J F
出版信息
J Bacteriol. 1974 Feb;117(2):747-52. doi: 10.1128/jb.117.2.747-752.1974.
Abstract
Hfr strains B4 and B8 transfer the Escherichia coli chromosome in opposite directions, each transferring lac(+) as the last known marker. They were mated in concurrent crosses with the proA leu metE lys trp purE lac strain chi462. Analysis of the time of entry values for these markers showed that Hfr strain B8 transfers the whole chromosome more rapidly than does Hfr strain B4. In both crosses, the rate of transfer observed decelerates. If deceleration occurs as a function of the amount of chromosome transferred, the data are consistent with the markers examined being very accurately placed on the Taylor-Trotter map of the E. coli K-12 genome.
摘要
高频重组（Hfr）菌株B4和B8以相反方向转移大肠杆菌染色体，每个菌株都将乳糖发酵基因（lac+）作为最后一个已知标记进行转移。它们与proA leu metE lys trp purE lac菌株chi462同时进行杂交。对这些标记的进入时间值进行分析表明，Hfr菌株B8比Hfr菌株B4更快地转移整个染色体。在这两个杂交中，观察到的转移速率都在下降。如果转移速率下降是已转移染色体数量的函数，那么这些数据与所检测的标记在大肠杆菌K-12基因组的泰勒-特罗特图谱上定位非常准确这一情况是一致的。
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