Hercules K, Sauerbier W
J Virol. 1973 Oct;12(4):872-81. doi: 10.1128/JVI.12.4.872-881.1973.
We have investigated the possibility of assigning genes of T4 bacteriophage to their units of transcription (scriptons) by studying gene expression from UV-irradiated DNA templates. Since RNA chains are prematurely terminated on UV-irradiated DNA templates and since the promotor distal part of the RNA chain is deleted, the expression of any gene is inversely proportional to the distance between the promotor and the promotor distal end of the gene. We find that the early genes, 43, 45 and rIIB, are promotor proximal. Since at least genes 43 and rIIB are classified as delayed early genes, these results suggest that their synthesis may require the recognition of new promotors. Additional early genes (44, 62, 42, 46, 47, 55, and rIIA) and some late genes (34, 37, and 38) have also been assigned positions relative to their promotors.
我们通过研究紫外线照射的DNA模板上的基因表达，探讨了将T4噬菌体基因定位到其转录单位（转录子）的可能性。由于RNA链在紫外线照射的DNA模板上会提前终止，且RNA链启动子远端部分会缺失，所以任何基因的表达与启动子和该基因启动子远端之间的距离成反比。我们发现早期基因43、45和rIIB位于启动子近端。由于至少基因43和rIIB被归类为延迟早期基因，这些结果表明它们的合成可能需要识别新的启动子。其他早期基因（44、62、42、46、47、55和rIIA）以及一些晚期基因（34、37和38）也已被定位到相对于其启动子的位置。
