噬菌体T7中的转录单位图谱。I. 大肠杆菌RNA聚合酶的体内转录
Transcription unit mapping in bacteriophage T7. I. In vivo transcription by Escherichia coli RNA polymerase.
作者信息
Bräutigam A R, Sauerbier W
出版信息
J Virol. 1973 Oct;12(4):882-6. doi: 10.1128/JVI.12.4.882-886.1973.
Abstract
Premature termination of transcription at UV lesions in DNA permits a study of the sequential order of transcription by E. coli RNA polymerase of the genes in the early region of T7. This analysis is extended to the transcription by E. coli polymerase of the late region of T7 by deleting the early terminator. The results demonstrate the existence of a single large transcription unit spanning the early region, with a promotor located at the left end of the T7 genome. Furthermore, there are no initiation sites for E. coli RNA polymerase in the late region. When E. coli polymerase transcribes the late region, it does so exclusively by initiation at the early promotor.
摘要
DNA中紫外线损伤处转录的过早终止，使得对T7早期区域基因的大肠杆菌RNA聚合酶转录顺序进行研究成为可能。通过删除早期终止子，将该分析扩展至大肠杆菌聚合酶对T7晚期区域的转录。结果表明，存在一个跨越早期区域的单一大型转录单元，其启动子位于T7基因组的左端。此外，晚期区域不存在大肠杆菌RNA聚合酶的起始位点。当大肠杆菌聚合酶转录晚期区域时，它完全通过在早期启动子处起始来进行转录。
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