Shevach E M, Paul W E, Green I
J Exp Med. 1974 Mar 1;139(3):661-78. doi: 10.1084/jem.139.3.661.
It has been previously shown that alloantisera prepared by reciprocal immunization of strain 2 and strain 13 guinea pigs specifically block the activation of T lymphocytes from immune guinea pigs by antigens, the response to which is controlled by Ir genes. In this report we have examined the effect of absorption of the 13 anti-2 serum with different populations of lymphoid cells. It is unlikely that the inhibitory activity of the anti-2 serum on the proliferation of (2 x 13)F(1) lymphocytes to a DNP derivative of a copolymer of L-glutamic and L-lysine (DNP-GL) is due to the presence of antibodies specific for the unique antigenic determinants (idiotypes) of clonally distributed T-lymphocyte receptors. Thus, cells obtained from a normal animal and a DNP-GL immune animal were equivalent in their absorptive capacity. Populations of T lymphocytes were ineffective in absorbing either the cytotoxic or inhibitory activity of the anti-2 serum, while L(2)C leukemia cells, a malignant B-cell population, were most efficient in absorbing both activities. Thus, the antigen(s) against which the cytotoxic and inhibitory activities are directed are present to a greater extent on B lymphocytes than on T lymphocytes. However, these results do not allow us to definitively determine whether the inhibitory activity of the alloantisera is due to antibodies specific for Ir gene products or antibodies specific for linked antigens in the MHC. We also examined the effect of a number of anti-immunoglobulin reagents which had specificity for the heavy and/or light chains of guinea pig immunoglobulin on the in vitro lymphocyte proliferative response to antigen. Under conditions in which we were able to completely and specifically suppress the response of (2 x 13)F(1) lymphocytes to DNP-GL with anti-2 serum, the anti-immunoglobulin reagents were devoid of inhibitory effect on the response of these same F(1) cells to DNP-GL, a copolymer of L-glutamic and L-tyrosine (GT), or purified protein derivative of tuberculin (PPD). These results strongly suggest that conventional serum-type immunoglobulin is not important in antigen recognition by the T cells involved in the DNA synthetic response.
先前的研究表明，通过2系和13系豚鼠相互免疫制备的同种抗血清能特异性阻断免疫豚鼠的T淋巴细胞被抗原激活，而对该抗原的反应受Ir基因控制。在本报告中，我们检测了用不同淋巴细胞群体吸收13抗2血清的效果。抗2血清对（2×13）F1淋巴细胞对L-谷氨酸和L-赖氨酸共聚物的DNP衍生物（DNP-GL）增殖的抑制活性，不太可能是由于存在针对克隆分布的T淋巴细胞受体独特抗原决定簇（独特型）的特异性抗体。因此，从正常动物和DNP-GL免疫动物获得的细胞在吸收能力上是相当的。T淋巴细胞群体在吸收抗2血清的细胞毒性或抑制活性方面无效，而L(2)C白血病细胞，一种恶性B细胞群体，在吸收这两种活性方面最有效。因此，针对其细胞毒性和抑制活性的抗原在B淋巴细胞上的存在程度比在T淋巴细胞上更大。然而，这些结果并不能让我们明确确定同种抗血清的抑制活性是由于针对Ir基因产物的特异性抗体还是针对MHC中连锁抗原的特异性抗体。我们还检测了一些对豚鼠免疫球蛋白重链和/或轻链具有特异性的抗免疫球蛋白试剂对体外淋巴细胞对抗原增殖反应的影响。在我们能够用抗2血清完全特异性抑制（2×13）F1淋巴细胞对DNP-GL反应的条件下，抗免疫球蛋白试剂对这些相同F1细胞对DNP-GL、L-谷氨酸和L-酪氨酸共聚物（GT）或结核菌素纯蛋白衍生物（PPD）的反应没有抑制作用。这些结果强烈表明，传统血清型免疫球蛋白在参与DNA合成反应的T细胞抗原识别中并不重要。
