Smith J T, Wyatt J M
J Bacteriol. 1974 Mar;117(3):931-9. doi: 10.1128/jb.117.3.931-939.1974.
The release of several R factor and chromosomal beta-lactamases by osmotic shock treatment was studied. It was found that those beta-lactamases with a molecular weight of about 20,000 were released, but those with a molecular weight of about 30,000 to 44,000 were not released during osmotic shock. This differential release did not depend on whether the structural genes were on the chromosome or on the genome of an R factor. The release or retention of the beta-lactamases appeared to be a characteristic of the enzyme rather than the host cell since the same results were obtained when the R factors were harbored by a variety of host bacteria. Studies with bacteria which produced more than one beta-lactamase showed that each enzyme reacted independently to the presence of other beta-lactamases produced by the host bacterium.
研究了通过渗透休克处理释放几种R因子和染色体β-内酰胺酶的情况。结果发现，分子量约为20,000的那些β-内酰胺酶会被释放，但分子量约为30,000至44,000的那些β-内酰胺酶在渗透休克期间不会被释放。这种差异释放并不取决于结构基因是位于染色体上还是R因子的基因组上。β-内酰胺酶的释放或保留似乎是酶的特性而非宿主细胞的特性，因为当多种宿主细菌携带R因子时会得到相同的结果。对产生不止一种β-内酰胺酶的细菌进行的研究表明，每种酶对宿主细菌产生的其他β-内酰胺酶的存在反应是独立的。
