Murphy J W, Gregory J A, Larsh H W
Infect Immun. 1974 Feb;9(2):404-9. doi: 10.1128/iai.9.2.404-409.1974.
This study was undertaken to evaluate the potential of a cryptococcal culture filtrate antigen, cryptococcin C184, for detecting delayed hypersensitivity in Cryptococcus neoformans-injected animals. The antigen was tested on guinea pigs which had received saline or C. neoformans and on animals sensitized to Histoplasma capsulatum, Blastomyces dermatitidis, Candida albicans, or Sporothrix schenckii. A delayed-type hypersensitivity response was elicited by cryptococcin C184 in C. neoformans-injected guinea pigs, whereas no indurations or erythemas were seen at 48 h after skin testing of saline controls or heterologously sensitized guinea pigs. Besides being specific for Cryptococcus, the antigen showed a high degree of sensitivity and was reproducible. Footpad tests were conducted with the antigen on mice which had previously received either 10(5) viable C. neoformans cells or saline. Delayed hypersensitivity was indicated in the C. neoformans-injected mice by the increase in thickness of antigen-injected footpads when compared with the saline-injected footpads. In control mice, antigen- and saline-injected footpads were comparable in thickness 24 h after injection. Mice sensitized to B. dermatitidis were footpad tested with C184, and no cross-reactivity was demonstrated.
本研究旨在评估新型隐球菌培养滤液抗原隐球菌素C184在检测新型隐球菌注射动物迟发型超敏反应中的潜力。该抗原在接受生理盐水或新型隐球菌注射的豚鼠以及对荚膜组织胞浆菌、皮炎芽生菌、白色念珠菌或申克孢子丝菌致敏的动物身上进行了测试。新型隐球菌素C184在注射新型隐球菌的豚鼠中引发了迟发型超敏反应，而在生理盐水对照组或异源致敏豚鼠皮肤试验后48小时未见硬结或红斑。除了对新型隐球菌具有特异性外，该抗原还表现出高度的敏感性且可重复。用该抗原对先前接受10(5)个活的新型隐球菌细胞或生理盐水注射的小鼠进行足垫试验。与注射生理盐水的足垫相比，注射新型隐球菌的小鼠中，注射抗原的足垫厚度增加表明存在迟发型超敏反应。在对照小鼠中，注射抗原和生理盐水的足垫在注射后24小时厚度相当。用C184对皮炎芽生菌致敏的小鼠进行足垫试验，未显示交叉反应。
