Murphy J W, Mosley R L, Cherniak R, Reyes G H, Kozel T R, Reiss E
Department of Botany-Microbiology, University of Oklahoma, Norman 73019.
Infect Immun. 1988 Feb;56(2):424-31. doi: 10.1128/iai.56.2.424-431.1988.
We compared a cryptococcal culture filtrate antigen referred to as CneF with chemically defined cryptococcal antigen fractions isolated by Cherniak and co-workers by using double immunodiffusion gels, polyacrylamide gel electrophoresis, immunoblots, and footpad reactivity of immunized mice. The three previously described components of cryptococcal culture filtrates are a high-molecular-weight glucuronoxylo-mannan (GXM), which is the major constituent, a galactoxylomannan (GaIXM), and a mannoprotein (MP). In this study we demonstrated that CneF contained components which were serologically and electrophoretically similar to the three previously described cryptococcal culture filtrate fractions. The MP fraction elicited significantly stronger delayed-type hypersensitivity responses than did the GXM or GaIXM fraction when used in mice immunized either with the CneF in complete Freund adjuvant or whole heat-killed Cryptococcus neoformans yeast cells. These findings were confirmed when the footpads of immunized mice were challenged with GaIXM and MP preparations from a culture filtrate of a C. neoformans acapsular mutant that does not produce GXM. Thus, we concluded that the MP was the primary component recognized by the anticryptococcal cell-mediated immune response in mice.
我们使用双免疫扩散凝胶、聚丙烯酰胺凝胶电泳、免疫印迹以及免疫小鼠的足垫反应性，将一种名为CneF的隐球菌培养滤液抗原与Cherniak及其同事分离得到的化学定义的隐球菌抗原组分进行了比较。隐球菌培养滤液先前描述的三种成分是高分子量葡糖醛酸木聚糖甘露聚糖（GXM），它是主要成分，半乳糖木聚糖甘露聚糖（GaIXM）和甘露糖蛋白（MP）。在本研究中，我们证明CneF所含的成分在血清学和电泳方面与先前描述的三种隐球菌培养滤液组分相似。当用完全弗氏佐剂中的CneF或全热灭活的新型隐球菌酵母细胞免疫小鼠时，MP组分引发的迟发型超敏反应比GXM或GaIXM组分明显更强。当用来自不产生GXM的新型隐球菌无荚膜突变体培养滤液的GaIXM和MP制剂攻击免疫小鼠的足垫时，这些发现得到了证实。因此，我们得出结论，MP是小鼠抗隐球菌细胞介导的免疫反应识别的主要成分。
