来自在低磷酸盐培养基中指数生长的大肠杆菌的苯丙氨酸转移核糖核酸的新色谱形式。
New chromatographic form of phenylalanine transfer ribonucleic acid from Escherichia coli growing exponentially in a low-phosphate medium.
作者信息
Mann M B, Huang P C
出版信息
J Bacteriol. 1974 Apr;118(1):209-12. doi: 10.1128/jb.118.1.209-212.1974.
Abstract
Reversed-phase chromatography has been used to detect the presence of a new form of phenylalanyl-transfer ribonucleic acid (Phe-tRNA) from Escherichia coli growing exponentially in media containing low but nonlimiting levels of inorganic phosphate. The amount of this extra Phe-tRNA form is greatest in slowly growing cells (0.8 generations/h), and becomes negligible in media supporting a rapid growth rate (2.14 generations/h).
摘要
反相色谱法已被用于检测在含有低但非限制性无机磷酸盐水平的培养基中指数生长的大肠杆菌中一种新形式的苯丙氨酰转移核糖核酸（Phe-tRNA）的存在。这种额外的Phe-tRNA形式的量在生长缓慢的细胞（0.8代/小时）中最大，而在支持快速生长速率（2.14代/小时）的培养基中变得可以忽略不计。
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