在松弛型大肠杆菌蛋氨酸饥饿期间，对转移核糖核酸中核苷Q形成的抑制作用。
Inhibition of nucleoside Q formation in transfer ribonucleic acid during methionine starvation of relaxed-control Escherichia coli.
作者信息
Katze J R, Mosteller R D
出版信息
J Bacteriol. 1976 Jan;125(1):205-10. doi: 10.1128/jb.125.1.205-210.1976.
Abstract
The elution profiles of Asp-tRNA from unstarved and starved cultures of a relaxed-control (Rel-) strain of Escherichia coli were compared by reversed-phase chromatography. Methionine starvation results in the appearance of several additional species of Asp-tRNA which are not observed with starvation for leucine or histidine. By the criterion of cyanogen bromide-effected shifts in chromatographic elution position, a large portion of the tRNAAsp synthesized in methionine-starved cells lacks the normal Q nucleoside. By the same criterion, virtually all of the tRNAAsp from unstarved, leucine-starved, and histidine-starved cells contain Q. We conclude that methionine starvation prevents the formation of the norma Q nucleoside in Rel- E. coli.
摘要
通过反相色谱法比较了来自大肠杆菌松弛控制（Rel-）菌株未饥饿和饥饿培养物中Asp-tRNA的洗脱图谱。甲硫氨酸饥饿导致出现几种额外的Asp-tRNA种类，而亮氨酸或组氨酸饥饿时未观察到这种情况。根据溴化氰引起的色谱洗脱位置变化的标准，在甲硫氨酸饥饿细胞中合成的大部分tRNAAsp缺乏正常的Q核苷。根据相同标准，来自未饥饿、亮氨酸饥饿和组氨酸饥饿细胞的几乎所有tRNAAsp都含有Q。我们得出结论，甲硫氨酸饥饿会阻止Rel-大肠杆菌中正常Q核苷的形成。
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