硫胺素或其单磷酸酯对大肠杆菌中硫胺素焦磷酸利用的抑制作用。
Inhibition of thiamine pyrophosphate utilization by thiamine or its monophosphate in Escherichia coli.
作者信息
Nakayama H, Hayashi R
出版信息
J Bacteriol. 1974 Apr;118(1):32-40. doi: 10.1128/jb.118.1.32-40.1974.
The growth of a thiamine pyrophosphate auxotroph of Escherichi coli was inhibited by either thiamine or thiamine monophosphate, and the growth of a thiamine monophosphate auxotroph was inhibited by thiamine. The thiamine pyrophosphate-dependent oxidation of pyruvate was inhibited by thiamine with whole cells of the thiamine pyrophosphate auxotroph but not with cell extracts prepared from the same organism. In addition, the thiamine pyrophosphate uptake of the thiamine pyrophosphate auxotroph was inhibited by either thiamine or thiamine monophosphate. Although the thiamine pyrophosphate uptake of a revertant, selected for prototrophy from the thiamine monophosphate auxotroph, was inhibited by thiamine to an extent comparable to that observed with the thiamine monophosphate auxotroph, its growth was no longer inhibited by thiamine. A possible mechanism for the inhibition by thiamine and thiamine monophosphate in the utilization of thiamine pyrophosphate is discussed.
硫胺素焦磷酸营养缺陷型大肠杆菌的生长会被硫胺素或硫胺素单磷酸抑制，而硫胺素单磷酸营养缺陷型的生长会被硫胺素抑制。硫胺素焦磷酸营养缺陷型的完整细胞中，硫胺素会抑制丙酮酸依赖硫胺素焦磷酸的氧化，但从同一生物体制备的细胞提取物中则不会。此外，硫胺素焦磷酸营养缺陷型对硫胺素焦磷酸的摄取会被硫胺素或硫胺素单磷酸抑制。虽然从硫胺素单磷酸营养缺陷型中选择的原养型回复突变体对硫胺素焦磷酸的摄取被硫胺素抑制的程度与硫胺素单磷酸营养缺陷型相当，但其生长不再被硫胺素抑制。本文讨论了硫胺素和硫胺素单磷酸在硫胺素焦磷酸利用中产生抑制作用的一种可能机制。
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