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E型肉毒梭菌营养细胞的热损伤与恢复

Heat injury and recovery of vegetative cells of Clostridium botulinum type E.

作者信息

Pierson M D, Payne S L, Ades G L

出版信息

Appl Microbiol. 1974 Feb;27(2):425-6. doi: 10.1128/am.27.2.425-426.1974.

Abstract

Vegetative cells of Clostridium botulinum type E (Tenno) were heated at 40.5 C in a prereduced peptone-yeast extract broth (PY). During the heating period, cell numbers remained essentially constant for 3 h as indicated by roll tube counts in PY agar (PYA); however, injury and recovery from injury were observed when the cells were enumerated using PYA containing either 0.06 or 0.07% bile salts.

摘要

E型肉毒梭菌(Tenno)的营养细胞在预先还原的蛋白胨-酵母提取物肉汤(PY)中于40.5℃加热。在加热期间,如PY琼脂(PYA)中的滚管计数所示,细胞数量在3小时内基本保持恒定;然而,当使用含有0.06%或0.07%胆盐的PYA对细胞进行计数时,观察到细胞损伤及损伤后的恢复情况。

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