Pierson M D, Payne S L, Ades G L
Appl Microbiol. 1974 Feb;27(2):425-6. doi: 10.1128/am.27.2.425-426.1974.
Vegetative cells of Clostridium botulinum type E (Tenno) were heated at 40.5 C in a prereduced peptone-yeast extract broth (PY). During the heating period, cell numbers remained essentially constant for 3 h as indicated by roll tube counts in PY agar (PYA); however, injury and recovery from injury were observed when the cells were enumerated using PYA containing either 0.06 or 0.07% bile salts.
E型肉毒梭菌（Tenno）的营养细胞在预先还原的蛋白胨-酵母提取物肉汤（PY）中于40.5℃加热。在加热期间，如PY琼脂（PYA）中的滚管计数所示，细胞数量在3小时内基本保持恒定；然而，当使用含有0.06%或0.07%胆盐的PYA对细胞进行计数时，观察到细胞损伤及损伤后的恢复情况。
