Klein D A, Wu S
Appl Microbiol. 1974 Feb;27(2):429-31. doi: 10.1128/am.27.2.429-431.1974.
Heterotrophic microorganisms in water samples are susceptible to the transient stress of warmed agar used in the standard methods pour plate procedure, causing significantly decreased recoveries in comparison with a spread plate technique. Microbial starvation can increase susceptibility to a transient warming stress. The standard plate count procedure, as presently described, should not be considered for quantitation of microorganisms from aquatic environments.
水样中的异养微生物易受标准方法倾注平板程序中使用的温热琼脂的短暂应激影响,与涂布平板技术相比,回收率显著降低。微生物饥饿会增加对短暂温热应激的敏感性。目前所述的标准平板计数程序不适用于对水生环境中的微生物进行定量分析。