关于人类病原体申克孢子丝菌和长喙壳菌属物种的脱氧核糖核酸碱基组成及杂交研究
Deoxyribonucleic acid base composition and hybridization studies on the human pathogen Sporothrix schenckii and Ceratocystis species.
作者信息
Mendonça-Hagler L C, Travassos L R, Lloyd K O, Phaff H J
出版信息
Infect Immun. 1974 May;9(5):934-8. doi: 10.1128/iai.9.5.934-938.1974.
Abstract
Deoxyribonucleic acid (DNA) base composition, as measured by guanine plus cytosine (G + C) content, was determined in 17 strains of Sporothrix schenckii and Ceratocystis species. The average G + C content for S. schenckii was 54.7 +/- 0.2 mol%. No strain of Ceratocystis, with the exception of C. minor, gave values for G + C content similar to those obtained for S. schenckii. All DNA samples from strains of S. schenckii cross-hybridized with high percentages of relative binding. DNA samples from C. stenoceras and C. pilifera showed low degrees of homology with S. schenckii DNA. The DNA from C. minor hybridized with 75% binding with that from a strain of S. schenckii, as compared with the homologous reaction.
摘要
通过鸟嘌呤加胞嘧啶（G + C）含量测定了17株申克孢子丝菌和长喙壳菌属物种的脱氧核糖核酸（DNA）碱基组成。申克孢子丝菌的平均G + C含量为54.7 +/- 0.2摩尔％。除小长喙壳菌外，没有长喙壳菌属菌株的G + C含量值与申克孢子丝菌的相似。来自申克孢子丝菌菌株的所有DNA样品均以高比例的相对结合进行交叉杂交。窄长喙壳菌和毛长喙壳菌的DNA样品与申克孢子丝菌DNA的同源性较低。与同源反应相比，小长喙壳菌的DNA与一株申克孢子丝菌的DNA以75％的结合率杂交。
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