Fay G D, Barry A L
Appl Microbiol. 1974 Mar;27(3):562-5. doi: 10.1128/am.27.3.562-565.1974.
To help select the most appropriate method for detecting indole production with anaerobic gram-negative bacilli, several recommended methods were compared. Indole was measured both quantitatively and qualitatively after varying periods of incubation. Studies evaluated the results obtained in different media, the effect of adding glucose and/or tryptophan, the requirement for strict anaerobiasis, and the effects of reducing the total volume of broth. A 1-ml amount of thioglycolate broth without glucose but with 0.02% tryptophan gave optimal results after 2 to 7 days of incubation in anaerobic (Gas-Pak) jars. The majority of clinical isolates will give strong positive tests after 1 to 2 days but a few require 3 to 7 days of incubation. Prolonged incubation was required more frequently with conventional methods.
为了帮助选择检测厌氧革兰氏阴性杆菌吲哚产生的最合适方法，对几种推荐方法进行了比较。在不同的孵育时间后，对吲哚进行了定量和定性测量。研究评估了在不同培养基中获得的结果、添加葡萄糖和/或色氨酸的效果、严格厌氧的要求以及减少肉汤总体积的影响。1毫升不含葡萄糖但含有0.02%色氨酸的硫乙醇酸盐肉汤，在厌氧（气体Pak）罐中孵育2至7天后可获得最佳结果。大多数临床分离株在1至2天后会给出强阳性测试结果，但少数需要3至7天的孵育时间。传统方法更频繁地需要延长孵育时间。
