Wideman P A, Citronbaum D M, Sutter V L
J Clin Microbiol. 1977 Mar;5(3):315-9. doi: 10.1128/jcm.5.3.315-319.1977.
The laboratory and clinical evaluation of a potassium nitrate-saturated disk for the rapid detection of nitrate reductase production in anaerobes was investigated. The optimal disk concentration and incubation time were determined by utilizing triplicate sets of quadrant plates prepared with supplemented brucella (Difco) blood agar and swabbed with a 24-h broth (BBL; 135 C thioglycolate) suspension of the test organism. Each set of plates received one control disk and three disks of varying concentrations of potassium nitrate (1 to 8 mg) with 0.1% sodium molybdate. All sets were incubated in GasPak jars for 24, 48, or 72 h, and subsequently sulfanilic acid and 1,6-Cleve's acid were added to each disk. A pink or red color change was indicative of nitrate reductase production. Eighty-eight stock isolates, 23 American Type Culture Collection strains, and 214 fresh clinical isolates were evaluated and compared with results obtained with tubes of preduced indole-nitrite medium (BBL) incubated for 7 to 10 days. The 6-mg disk incubated for 48 h yielded an overall agreement of 89% with the conventional tube technique, and fresh clinical isolates demonstrated better disk-tube agreement (93%) than previously frozen stock strains. The simplicity and ease of this disk test suggest its value as a preliminary screening procedure for nitrate reductase production. There were no false positives. Negative results by disk should be rechecked by tube.
对一种用于快速检测厌氧菌中硝酸还原酶产生的硝酸钾饱和圆盘进行了实验室和临床评估。通过使用一式三份的象限平板来确定最佳圆盘浓度和孵育时间，这些平板用补充了布鲁氏菌（Difco）血琼脂制备，并接种测试微生物的24小时肉汤（BBL；135 C硫乙醇酸盐）悬液。每组平板接受一个对照圆盘和三个不同浓度（1至8毫克）的硝酸钾圆盘，并添加0.1%的钼酸钠。所有组在GasPak罐中孵育24、48或72小时，随后向每个圆盘添加磺胺酸和1,6 - 克利夫酸。粉红色或红色变化表明产生了硝酸还原酶。对88株库存菌株、23株美国典型培养物保藏中心菌株和214株新鲜临床分离株进行了评估，并与在预还原吲哚 - 亚硝酸盐培养基（BBL）管中孵育7至10天所获得的结果进行比较。孵育48小时的6毫克圆盘与传统管法的总体一致性为89%，新鲜临床分离株的圆盘 - 管一致性（93%）比先前冷冻的库存菌株更好。这种圆盘试验的简单性和易用性表明其作为硝酸还原酶产生的初步筛选程序的价值。没有假阳性。圆盘试验的阴性结果应通过管法重新检查。
