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用酶桥法检测自身抗体。

The detection of autoantibodies with an enzyme bridge method.

作者信息

Ford P M, Stoward P J

出版信息

J Clin Pathol. 1974 Feb;27(2):118-21. doi: 10.1136/jcp.27.2.118.

DOI:10.1136/jcp.27.2.118
PMID:4596415
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC478018/
Abstract

The horseradish peroxidase bridge method described by Mason, Phifer, Spicer, Swallow, and Dreskin (1969) has been found to be as sensitive and specific as fluorescence methods for detecting antinuclear factor and gastric parietal cell antibody, and to a lesser extent, antibodies to smooth muscle and mitochondria. However, the method has at least four practical advantages over fluorescence techniques.

摘要

梅森、菲弗、斯派塞、斯沃洛和德雷斯金(1969年)描述的辣根过氧化物酶桥法已被发现,在检测抗核因子和胃壁细胞抗体方面与荧光法一样灵敏和特异,在检测平滑肌和线粒体抗体方面灵敏度稍低。然而,该方法相对于荧光技术至少有四个实际优势。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2dee/478018/16d0077f1c63/jclinpath00124-0034-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2dee/478018/457d604f07a6/jclinpath00124-0033-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2dee/478018/db8600a55816/jclinpath00124-0034-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2dee/478018/16d0077f1c63/jclinpath00124-0034-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2dee/478018/457d604f07a6/jclinpath00124-0033-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2dee/478018/db8600a55816/jclinpath00124-0034-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2dee/478018/16d0077f1c63/jclinpath00124-0034-b.jpg

相似文献

1
The detection of autoantibodies with an enzyme bridge method.用酶桥法检测自身抗体。
J Clin Pathol. 1974 Feb;27(2):118-21. doi: 10.1136/jcp.27.2.118.
2
Smooth muscle antibody in patients with warts.疣患者体内的平滑肌抗体。
Clin Exp Immunol. 1975 Aug;21(2):339-44.
3
Peroxidase conjugates for demonstration of tissue antibodies: evaluation of the technique.用于显示组织抗体的过氧化物酶结合物:技术评估
Clin Exp Immunol. 1971 Sep;9(3):407-18.
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[Determination of autoantibodies by immunofluorescence in hepatic pathology].[肝脏病理学中通过免疫荧光法检测自身抗体]
Acta Gastroenterol Latinoam. 1973 Jan-Mar;5(1):13-7.
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[Anti-tissue antibodies in human pathology].[人类病理学中的抗组织抗体]
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Smooth muscle antibody in bronchial asthma.支气管哮喘中的平滑肌抗体
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Indirect immunofluorescence studies of smooth muscle antibodies.平滑肌抗体的间接免疫荧光研究。
Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Aug;82(4):577-84. doi: 10.1111/j.1699-0463.1974.tb02368.x.
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Australia antigen and autoantibodies in chronic hepatitis.慢性肝炎中的澳大利亚抗原和自身抗体
Br Med J. 1970 Jun 20;2(5711):695-8. doi: 10.1136/bmj.2.5711.695.
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[Laboratory studies in alveolitis and pulmonary fibrosis (with particular regard to immunologic methods].
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引用本文的文献

1
The localization of enzymes in tissue sections by immuno-histochemistry. Conventional antibody and mixed aggregation techniques.通过免疫组织化学对组织切片中的酶进行定位。传统抗体和混合聚集技术。
Histochem J. 1976 May;8(3):253-70. doi: 10.1007/BF01003815.

本文引用的文献

1
The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.注入的辣根过氧化物酶在小鼠肾近端小管吸收的早期阶段:一种新技术的超微结构细胞化学研究
J Histochem Cytochem. 1966 Apr;14(4):291-302. doi: 10.1177/14.4.291.
2
Development of chicken lymphoid system. II. Synthesis of primordial immunoglobulin M by the bursa cells of chick embryo.鸡淋巴系统的发育。II. 鸡胚法氏囊细胞合成原始免疫球蛋白M
J Exp Med. 1972 Jul 1;136(1):8-20. doi: 10.1084/jem.136.1.8.
3
Ultrastructural localization of heterologous nephrotoxic antibody in the glomerular basement membrane of the rat.
异种肾毒性抗体在大鼠肾小球基底膜中的超微结构定位
Lab Invest. 1972 May;26(5):610-3.
4
An immunoglobulin-enzyme bridge method for localizing tissue antigens.一种用于定位组织抗原的免疫球蛋白 - 酶桥法。
J Histochem Cytochem. 1969 Sep;17(9):563-9. doi: 10.1177/17.9.563.
5
Immunocytologic labeling of calf and human lymphocyte surface antigens.小牛和人类淋巴细胞表面抗原的免疫细胞化学标记
Lab Invest. 1971 Sep;25(3):211-9.