Ault K A, Unanue E R
J Exp Med. 1974 May 1;139(5):1110-24. doi: 10.1084/jem.139.5.1110.
The behavior of the immunoglobulin antigen receptor on lymphocytes was studied using both fluorescent antiimmunoglobulin antibody to detect B cells and autoradiography with radiolabeled antigens to detect antigen-binding cells. It was shown that after binding of antiimmunoglobulin antibody to the lymphocyte there was a rapid loss of surface immunoglobulin and then a progressive reappearance over 18 h. This could be quantitated using an inhibition assay for surface immunoglobulin. Similarly, after binding various dinitrophenyl-conjugated proteins or keyhole limpet hemocyanin to their specific antigen-binding cells, there was a loss of the antigen receptor from the surface and then a progressive reappearance of the receptor. The reappearance of surface immunoglobulin and of the antigen receptor proceeded at about the same rate. Repeated exposure to antibody or prolonged exposure to antigen did not diminish the capacity of the lymphocyte to re-express its receptor. These events, which follow the interaction of antigen and its receptor, are of possible importance in understanding the mechanism of triggering of the immune response and of tolerance.
利用荧光抗免疫球蛋白抗体检测B细胞，并通过放射性标记抗原的放射自显影术检测抗原结合细胞，对淋巴细胞上免疫球蛋白抗原受体的行为进行了研究。结果表明，抗免疫球蛋白抗体与淋巴细胞结合后，表面免疫球蛋白迅速丢失，然后在18小时内逐渐重新出现。这可以通过表面免疫球蛋白的抑制试验进行定量。同样，在各种二硝基苯基偶联蛋白或钥孔戚血蓝蛋白与其特异性抗原结合细胞结合后，表面的抗原受体丢失，然后受体逐渐重新出现。表面免疫球蛋白和抗原受体的重新出现速率大致相同。反复接触抗体或长时间接触抗原并不会削弱淋巴细胞重新表达其受体的能力。这些在抗原与其受体相互作用之后发生的事件，对于理解免疫反应触发机制和耐受性可能具有重要意义。
