脂质在大肠杆菌细胞包膜的细胞壁和细胞质膜亚组分中的分布。
Distribution of lipids in the wall and cytoplasmic membrane subfractions of the cell envelope of Escherichia coli.
作者信息
White D A, Lennarz W J, Schnaitman C A
出版信息
J Bacteriol. 1972 Feb;109(2):686-90. doi: 10.1128/jb.109.2.686-690.1972.
Abstract
Cell wall and membrane subfractions of the cell envelope of Escherichia coli have been isolated by a procedure involving particle electrophoresis and sucrose gradient density centrifugation. The lipid content of each fraction has been investigated. The individual phospholipids of both fractions are quantitatively similar except that the proportion of lysophosphatidylethanolamine is greater in the wall than in the membrane. Fatty acid analysis of the phospholipids of each fraction revealed that the wall phospholipids contain a greater proportion of palmitic acid. Coenzyme Q is almost exclusively localized in the cell membrane.
摘要
通过涉及颗粒电泳和蔗糖梯度密度离心的方法，已分离出大肠杆菌细胞包膜的细胞壁和细胞膜亚组分。对每个组分的脂质含量进行了研究。两个组分的各个磷脂在数量上相似，只是溶血磷脂酰乙醇胺在细胞壁中的比例高于细胞膜。对每个组分的磷脂进行脂肪酸分析表明，细胞壁磷脂中棕榈酸的比例更高。辅酶Q几乎完全定位于细胞膜中。
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