核糖体洗脱因子对氯霉素抑制作用的影响。
Effect of ribosomal wash factors on inhibition by chloramphenicol.
作者信息
Cameron H J, Rogers S M, Julian G R
出版信息
Antimicrob Agents Chemother. 1972 Sep;2(3):156-8. doi: 10.1128/AAC.2.3.156.
Abstract
In vitro protein-synthesizing systems from Escherichia coli can be categorized as either chloramphenicol-sensitive or chloramphenicol-insensitive. The chloramphenicol-sensitive systems used in this study required the presence of factors removed from ribosomes with 1.0 m NH(4)Cl when chromatographically purified ribosomes were used for amino acid incorporation. These ribosomal wash factors inhibited but did not eliminate amino acid incorporation in chloramphenicol-insensitive systems. For both systems, addition of increasing amounts of the ribosomal wash factors increased the sensitivity to chloramphenicol inhibition.
摘要
来自大肠杆菌的体外蛋白质合成系统可分为对氯霉素敏感型或对氯霉素不敏感型。本研究中使用的对氯霉素敏感的系统，当使用经色谱纯化的核糖体进行氨基酸掺入时，需要存在用1.0 m NH₄Cl从核糖体中去除的因子。这些核糖体洗脱因子抑制但并未消除对氯霉素不敏感系统中的氨基酸掺入。对于这两种系统，添加越来越多的核糖体洗脱因子都会增加对氯霉素抑制的敏感性。
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本文引用的文献
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(14C)LYSINE PEPTIDES SYNTHESIZED IN AN IN VITRO ESCHERICHIA COLI SYSTEM IN THE PRESENCE OF CHLORAMPHENICOL.在氯霉素存在的情况下，在体外大肠杆菌系统中合成的（14C）赖氨酸肽。
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The role of the native subribosomal particles of Escherichia coli in polypeptide chain initiation.大肠杆菌天然亚核糖体颗粒在多肽链起始过程中的作用。
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