Brown G C, O'Leary T P
Infect Immun. 1974 Jun;9(6):1098-101. doi: 10.1128/iai.9.6.1098-1101.1974.
Paired sera from clinical cases, familial contacts, and school contacts of hand, foot, and mouth disease were tested by indirect immunofluorescence against a strain of coxsackie A16 virus which had been adapted to human fibroblast tissue-cultured cells. All of the 22 clinical cases developed immunoglobulin (Ig) G antibodies, and 11 responded with IgM and 10 with IgA antibodies. Seventeen of 21 familial contacts of cases showed subclinical infection as determined by development of IgG antibodies. Only seven and nine, respectively, of these had demonstrable IgM and IgA antibodies. Thirteen of 16 school classmates of three clinical cases were shown to have experienced subclinical infection by the development of IgG antibodies. Only five had antibodies in the IgM fraction, and three had antibodies in the IgA fraction. A comparison of IgG titers with those obtained by neutralization tests provides further evidence that the indirect fluorescent antibody technique represents a rapid diagnostic procedure for this disease.
采用间接免疫荧光法，以一株适应于人成纤维细胞组织培养细胞的柯萨奇A16病毒，对手足口病临床病例、家庭接触者和学校接触者的配对血清进行检测。22例临床病例均产生了免疫球蛋白（Ig）G抗体，11例产生IgM抗体，10例产生IgA抗体。21例病例的家庭接触者中，有17例通过产生IgG抗体被判定为亚临床感染。其中分别只有7例和9例有可检测到的IgM和IgA抗体。3例临床病例的16名学校同学中，有13例通过产生IgG抗体被证明经历过亚临床感染。只有5例有IgM组分抗体，3例有IgA组分抗体。将IgG滴度与中和试验所得结果进行比较，进一步证明间接荧光抗体技术是诊断该疾病的一种快速诊断方法。
