大肠杆菌素E3处理的核糖体在起始和链延伸中的活性。
Activity of colicin E3-treated ribosomes in initiation and in chain elongation.
作者信息
Davis B D
出版信息
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1021-5. doi: 10.1073/pnas.71.4.1021.
Abstract
Colicin E3 (called E3 here) is shown to act on polysomal ribosomes as well as on free ribosomes. This treatment severely inhibits subsequent chain elongation, both on preformed polysomes completing their nascent chains and on ribosomes translating poly(U). Elevated Mg(++) concentration relieves this inhibition. However, elevated Mg(++) concentration does not relieve the inhibition of ribosomes initiating on natural messenger; hence damage by E3 appears to have an additional, specific effect on some stage in initiation. The effect of damage by E3 resembles that of streptomycin in several respects. This similarity suggests that the two agents may act on closely related sites on the ribosome.
摘要
大肠杆菌素E3（此处称为E3）已被证明可作用于多聚核糖体以及游离核糖体。这种处理会严重抑制后续的链延伸，无论是在正在完成新生链合成的预先形成的多聚核糖体上，还是在翻译聚尿苷酸（poly(U)）的核糖体上。提高镁离子（Mg(++)）浓度可缓解这种抑制作用。然而，提高Mg(++)浓度并不能缓解对天然信使起始的核糖体的抑制作用；因此，E3造成的损伤似乎在起始的某个阶段具有额外的特定作用。E3造成的损伤在几个方面类似于链霉素的作用。这种相似性表明这两种药物可能作用于核糖体上密切相关的位点。
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本文引用的文献
1
STREPTOMYCIN, SUPPRESSION, AND THE CODE.链霉素、抑制作用与密码子
Proc Natl Acad Sci U S A. 1964 May;51(5):883-90. doi: 10.1073/pnas.51.5.883.
2
Colicin E3-induced in vitro inactivation of ribosomes from colicin-insensitive bacterial species.大肠杆菌素E3诱导对大肠杆菌素不敏感的细菌物种的核糖体在体外失活。
FEBS Lett. 1973 Jan 1;29(1):15-19. doi: 10.1016/0014-5793(73)80005-5.
3
Inactivation of ribosomes by colicin E3 in vitro: Requirement for 50 S ribosomal subunits.大肠杆菌素E3在体外使核糖体失活：对50S核糖体亚基的需求
FEBS Lett. 1972 Apr 15;22(1):73-75. doi: 10.1016/0014-5793(72)80222-9.
4
Streptomycin induced release of fMet-tRNA from the ribosomal initiation complex.链霉素诱导甲酰甲硫氨酸转运核糖核酸从核糖体起始复合物中释放出来。
Biochem Biophys Res Commun. 1971 Feb 5;42(3):530-7. doi: 10.1016/0006-291x(71)90403-7.
5
Interaction of colicins with bacterial cells. II. Specific alteration of Escherichia coli ribosomes induced by colicin E3 in vivo.大肠杆菌素与细菌细胞的相互作用。II. 体内大肠杆菌素E3诱导的大肠杆菌核糖体的特异性改变。
J Mol Biol. 1967 Jun 14;26(2):181-95. doi: 10.1016/0022-2836(67)90290-2.
6
The sedimentation behaviour of ribonuclease-active and -inactive ribosomes from bacteria.来自细菌的有核糖核酸酶活性和无核糖核酸酶活性的核糖体的沉降行为。
Biochem J. 1965 Sep;96(3):671-80. doi: 10.1042/bj0960671.
7
Inactivation of ribosomes in vitro by colicin E 3 .大肠杆菌素E3在体外使核糖体失活。
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Specific inactivation of 16S ribosomal RNA induced by colicin E3 in vivo.体内大肠菌素E3诱导的16S核糖体RNA的特异性失活
Proc Natl Acad Sci U S A. 1971 May;68(5):964-8. doi: 10.1073/pnas.68.5.964.
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Effect of colicin E3 upon the 30S ribosomal subunit of Escherichia coli.大肠杆菌素E3对大肠杆菌30S核糖体亚基的作用。
Proc Natl Acad Sci U S A. 1971 May;68(5):959-63. doi: 10.1073/pnas.68.5.959.
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J Mol Biol. 1970 Oct 28;53(2):205-20. doi: 10.1016/0022-2836(70)90295-0.
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