大肠杆菌素E3处理的核糖体在起始和链延伸中的活性。
Activity of colicin E3-treated ribosomes in initiation and in chain elongation.
作者信息
Davis B D
出版信息
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1021-5. doi: 10.1073/pnas.71.4.1021.
Abstract
Colicin E3 (called E3 here) is shown to act on polysomal ribosomes as well as on free ribosomes. This treatment severely inhibits subsequent chain elongation, both on preformed polysomes completing their nascent chains and on ribosomes translating poly(U). Elevated Mg(++) concentration relieves this inhibition. However, elevated Mg(++) concentration does not relieve the inhibition of ribosomes initiating on natural messenger; hence damage by E3 appears to have an additional, specific effect on some stage in initiation. The effect of damage by E3 resembles that of streptomycin in several respects. This similarity suggests that the two agents may act on closely related sites on the ribosome.
摘要
大肠杆菌素E3（此处称为E3）已被证明可作用于多聚核糖体以及游离核糖体。这种处理会严重抑制后续的链延伸，无论是在正在完成新生链合成的预先形成的多聚核糖体上，还是在翻译聚尿苷酸（poly(U)）的核糖体上。提高镁离子（Mg(++)）浓度可缓解这种抑制作用。然而，提高Mg(++)浓度并不能缓解对天然信使起始的核糖体的抑制作用；因此，E3造成的损伤似乎在起始的某个阶段具有额外的特定作用。E3造成的损伤在几个方面类似于链霉素的作用。这种相似性表明这两种药物可能作用于核糖体上密切相关的位点。
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