壮观霉素对起始核糖体的选择性抑制作用。
Selective inhibition of initiating ribosomes by spectinomycin.
作者信息
Wallace B J, Tai P C, Davis B D
出版信息
Proc Natl Acad Sci U S A. 1974 May;71(5):1634-8. doi: 10.1073/pnas.71.5.1634.
Abstract
Spectinomycin at low concentrations inhibits initiating ribosomes but not ribosomes already engaged in chain elongation. The initiating ribosomes are blocked in some step after formation of the initiation complex, probably the first translocation. Cells inhibited by spectinomycin accumulate polysomes, and these have proved to be unstable polyinitiation complexes: they can be pulse-labeled with methionine but not with valine, and they disappear after addition of rifampicin. Hence, the blocked ribosomes evidently are released and then reinitiate. In heterozygotes this cyclic reinitiation by the sensitive ribosomes, each blocking an initiation site for an average of 10-15 min, can explain (just as with streptomycin) the dominance of sensitivity over resistance.
摘要
低浓度的壮观霉素会抑制起始核糖体，但不会抑制已经参与链延伸的核糖体。起始核糖体在起始复合物形成后的某个步骤中被阻断，可能是第一次转位。受壮观霉素抑制的细胞会积累多核糖体，而这些多核糖体已被证明是不稳定的多起始复合物：它们可以用甲硫氨酸进行脉冲标记，但不能用缬氨酸标记，并且在加入利福平后会消失。因此，被阻断的核糖体显然会被释放，然后重新起始。在杂合子中，敏感核糖体的这种循环重新起始，每个核糖体平均阻断一个起始位点10 - 15分钟，这可以（就像链霉素一样）解释敏感性对抗性的显性现象。
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