乳糖阻遏蛋白模型及其与配体的相互作用。
Model for lactose repressor protein and its interaction with ligands.
作者信息
Dunaway M, Manly S P, Matthews K S
出版信息
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7181-5. doi: 10.1073/pnas.77.12.7181.
Abstract
A model is presented for the structure of the lactose repressor protein and for its interaction with inducer, operator DNA, and nonspecific DNA. The proposed structure is based on experimental evidence from this laboratory and from the literature and is offered as an integration of the available data on this system. Features unique to this model include: (i) interaction of the core region of the protein with the operator, (ii) primary effects of the conformational change in response to inducer on the core-operator interaction, (iii) contacts between all four subunits of the protein and the operator DNA, and (iv) qualitative differences in operator and nonspecific DNA binding.
摘要
本文提出了一种乳糖阻遏蛋白的结构模型，以及该蛋白与诱导剂、操纵基因DNA和非特异性DNA相互作用的模型。所提出的结构基于本实验室和文献中的实验证据，并作为该系统现有数据的整合呈现。该模型的独特特征包括：（i）蛋白质核心区域与操纵基因的相互作用；（ii）响应诱导剂时构象变化对核心-操纵基因相互作用的主要影响；（iii）蛋白质的所有四个亚基与操纵基因DNA之间的接触；以及（iv）操纵基因与非特异性DNA结合的质量差异。
相似文献
1
Model for lactose repressor protein and its interaction with ligands.乳糖阻遏蛋白模型及其与配体的相互作用。
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7181-5. doi: 10.1073/pnas.77.12.7181.
2
Equilibrium binding of inducer to lac repressor.operator DNA complex.诱导剂与乳糖阻遏蛋白-操纵基因DNA复合物的平衡结合
J Biol Chem. 1980 Nov 10;255(21):10107-14.
3
lac repressor: crystallization of intact tetramer and its complexes with inducer and operator DNA.乳糖阻遏蛋白：完整四聚体及其与诱导剂和操纵基因DNA复合物的结晶
Proc Natl Acad Sci U S A. 1990 Mar;87(5):1870-3. doi: 10.1073/pnas.87.5.1870.
4
lac repressor-lac operator interaction: NMR observations.乳糖阻遏蛋白-乳糖操纵子相互作用：核磁共振观察结果。
Proc Natl Acad Sci U S A. 1982 Jan;79(2):218-22. doi: 10.1073/pnas.79.2.218.
5
Enzymatic digestion of operator DNA in the presence of the lac repressor tryptic core.在乳糖阻遏物胰蛋白酶核心存在的情况下对操纵基因DNA进行酶切消化。
J Mol Biol. 1984 Nov 5;179(3):335-50. doi: 10.1016/0022-2836(84)90069-x.
6
Sequence-specific interactions of the tight-binding I12-X86 lac repressor with non-operator DNA.紧密结合的I12 - X86乳糖阻遏物与非操纵基因DNA的序列特异性相互作用。
Nucleic Acids Res. 1980 Feb 11;8(3):487-506. doi: 10.1093/nar/8.3.487.
7
lac operator DNA modification in the presence of proteolytic fragments of the repressor protein.在阻遏蛋白的蛋白水解片段存在的情况下，乳糖操纵子DNA修饰。
J Mol Biol. 1984 Nov 5;179(3):315-33. doi: 10.1016/0022-2836(84)90068-8.
8
Kinetic studies of inducer binding to lac repressor.operator complex.诱导剂与乳糖阻遏物-操纵基因复合物结合的动力学研究。
J Biol Chem. 1980 Nov 10;255(21):10115-9.
9
10
Lactose operator--repressor interaction: use of synthetic oligonucleotides in determining the minimal recognition sequence of the lactose operator.
Methods Enzymol. 1980;65(1):877-85. doi: 10.1016/s0076-6879(80)65080-0.
引用本文的文献
1
Broccoli aptamer allows quantitative transcription regulation studies in vitro.西兰花适配体可用于体外定量转录调控研究。
PLoS One. 2024 Jun 13;19(6):e0304677. doi: 10.1371/journal.pone.0304677. eCollection 2024.
2
Protein distributions from a stochastic model of the lac operon of E. coli with DNA looping: analytical solution and comparison with experiments.具有DNA环化的大肠杆菌乳糖操纵子随机模型的蛋白质分布：解析解及与实验的比较
PLoS One. 2014 Jul 23;9(7):e102580. doi: 10.1371/journal.pone.0102580. eCollection 2014.
3
Structure of the complex between lac repressor headpiece and operator DNA from measurements of the orientation relaxation and the electric dichroism.通过取向弛豫和电二色性测量得出的乳糖阻遏物头部片段与操纵基因DNA之间复合物的结构。
Nucleic Acids Res. 1982 Jun 25;10(12):3791-802. doi: 10.1093/nar/10.12.3791.
4
lac repressor-lac operator interaction: NMR observations.乳糖阻遏蛋白-乳糖操纵子相互作用：核磁共振观察结果。
Proc Natl Acad Sci U S A. 1982 Jan;79(2):218-22. doi: 10.1073/pnas.79.2.218.
5
Two helix DNA binding motif of CAP found in lac repressor and gal repressor.在乳糖阻遏物和半乳糖阻遏物中发现的CAP的双螺旋DNA结合基序。
Nucleic Acids Res. 1982 Aug 25;10(16):5085-102. doi: 10.1093/nar/10.16.5085.
6
Interaction between the lac operator and the lac repressor headpiece: fluorescence and circular dichroism studies.乳糖操纵基因与乳糖阻遏蛋白头部结构域之间的相互作用：荧光和圆二色性研究
EMBO J. 1982;1(11):1405-9. doi: 10.1002/j.1460-2075.1982.tb01330.x.
7
Quantitative model for gene regulation by lambda phage repressor.λ噬菌体阻遏物基因调控的定量模型。
Proc Natl Acad Sci U S A. 1982 Feb;79(4):1129-33. doi: 10.1073/pnas.79.4.1129.
8
Perturbation of lac operator DNA modification by tryptic core protein from lac repressor.来自乳糖阻遏物的胰蛋白酶核心蛋白对乳糖操纵子DNA修饰的干扰
Proc Natl Acad Sci U S A. 1983 Oct;80(20):6219-23. doi: 10.1073/pnas.80.20.6219.
9
Sequence of galR gene indicates a common evolutionary origin of lac and gal repressor in Escherichia coli.galR基因序列表明大肠杆菌中乳糖阻遏蛋白和半乳糖阻遏蛋白有共同的进化起源。
Proc Natl Acad Sci U S A. 1982 Apr;79(8):2427-31. doi: 10.1073/pnas.79.8.2427.
10
Lac repressor-Lac operator complexes. Solution X-ray scattering and electrophoretic studies.乳糖阻遏物 - 乳糖操纵子复合物。溶液X射线散射及电泳研究。
Eur Biophys J. 1987;14(3):169-78. doi: 10.1007/BF00253842.
本文引用的文献
1
An amino-terminal fragment of lac repressor binds specifically to lac operator.乳糖阻遏物的氨基端片段特异性结合乳糖操纵基因。
Proc Natl Acad Sci U S A. 1978 Dec;75(12):5851-4. doi: 10.1073/pnas.75.12.5851.
2
Sequence-specific interactions of the tight-binding I12-X86 lac repressor with non-operator DNA.紧密结合的I12 - X86乳糖阻遏物与非操纵基因DNA的序列特异性相互作用。
Nucleic Acids Res. 1980 Feb 11;8(3):487-506. doi: 10.1093/nar/8.3.487.
3
4
Kinetic studies of inducer binding to lac repressor.operator complex.诱导剂与乳糖阻遏物-操纵基因复合物结合的动力学研究。
J Biol Chem. 1980 Nov 10;255(21):10115-9.
5
Equilibrium binding of inducer to lac repressor.operator DNA complex.诱导剂与乳糖阻遏蛋白-操纵基因DNA复合物的平衡结合
J Biol Chem. 1980 Nov 10;255(21):10107-14.
6
DNA binding characteristics of lactose repressor and the trypsin-resistant core repressor.乳糖阻遏物和胰蛋白酶抗性核心阻遏物的DNA结合特性。
J Biol Chem. 1980 Nov 10;255(21):10100-6.
7
Neutron scattering studies of lac repressor.乳糖阻遏蛋白的中子散射研究。
Nature. 1980 Jul 24;286(5771):423-5. doi: 10.1038/286423a0.
8
Large-scale purification of two forms of active lac operator from plasmids.从质粒中大规模纯化两种活性乳糖操纵子形式。
Biochim Biophys Acta. 1980;606(1):113-24. doi: 10.1016/0005-2787(80)90103-3.
9
Ultraviolet difference spectra of the lactose repressor protein. II. Trypsin core protein.
Biochim Biophys Acta. 1974 Aug 8;359(2):334-40. doi: 10.1016/0005-2795(74)90232-3.
10
Genetic analysis of the active sites of lac repressor.乳糖阻遏物活性位点的遗传分析。
Genetics. 1974 Apr;76(4):669-79. doi: 10.1093/genetics/76.4.669.
Zotero 插件