酿酒酵母核糖体DNA在体内和体外的转录
Transcription of Saccharomyces cerevisiae ribosomal DNA in vivo and in vitro.
作者信息
Cramer J H, Sebastian J, Rownd R H, Halvorson H O
出版信息
Proc Natl Acad Sci U S A. 1974 Jun;71(6):2188-92. doi: 10.1073/pnas.71.6.2188.
In vivo, ribosomal RNA of Saccharomyces cerevisiae is transcribed from the light strand of gamma DNA. In vitro, gamma DNA is transcribed with equal efficiency by both of the yeast nuclear RNA polymerases, polymerase I and polymerase II; however the RNA products synthesized by the two enzymes differ from each other both in size and in the relative composition of guanine and cytosine. RNA synthesized by polymerase I hybridizes preferentially to the light strand, while that synthesized by polymerase II hybridizes equally well to either strand. Selective transcription of the light strand of the ribosomal DNA also occurs when high-molecular-weight total nuclear DNA is used as template.
在体内，酿酒酵母的核糖体RNA是从γDNA的轻链转录而来。在体外，γDNA被酵母的两种核RNA聚合酶，即聚合酶I和聚合酶II以相同的效率转录；然而，这两种酶合成的RNA产物在大小以及鸟嘌呤和胞嘧啶的相对组成上都彼此不同。由聚合酶I合成的RNA优先与轻链杂交，而由聚合酶II合成的RNA与两条链的杂交效果相同。当使用高分子量的总核DNA作为模板时，核糖体DNA轻链的选择性转录也会发生。
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