Tiktopulo E I, Privalov P L, Andreeva A P, Aleksandrov V Ia
Mol Biol (Mosk). 1979 May-Jun;13(3):619-24.
The method of hydrogen exchange is used to determine the mobility of acid-soluble collagen molecules from animals with different physiological temperature in terms of equilibrium constants of the formation of micro-unfolding or fluctuating defects of the structure. It has been shown that mobility of the collagen structure correlates with the physiological temperature of the animal from whose tissue collagen was isolated and is mainly determined by the amino acid content of the collagen. It has been shown also that at physiological temperatures characteristic for a particular species the level of collagen mobility is of the same order despite their different thermostability. The conclusion has been drawn that the level of mobility is the main criterion at the natural selection of the amino acid composition of collagens in different animals.
采用氢交换法，根据微展开或结构波动缺陷形成的平衡常数，来测定来自不同生理温度动物的酸溶性胶原分子的流动性。结果表明，胶原结构的流动性与分离胶原的动物的生理温度相关，且主要由胶原的氨基酸含量决定。还表明，在特定物种特有的生理温度下，尽管它们的热稳定性不同，但胶原流动性水平处于同一量级。由此得出结论，流动性水平是不同动物胶原氨基酸组成自然选择的主要标准。
