WATANABE T, FUKASAWA T
J Bacteriol. 1962 Apr;83(4):727-35. doi: 10.1128/jb.83.4.727-735.1962.
Watanabe, T. (Keio University, Tokyo, Japan) and T. Fukasawa. Episome-mediated transfer of drug resistance in Enterobacteriaceae. IV. Interactions between resistance transfer factor and F-factor in Escherichia coli K-12. J. Bacteriol. 83:727-735. 1962. - Resistance transfer factor (RTF) present in F(+) and F'(+) strains was found to suppress completely the transfer of F-factor, F', and host chromosomes. On the other hand, the presence of RTF in Hfr strains was found to reduce the frequencies of recombination to about 10(-2) of those in Hfr strains, regardless of the types of Hfr. RTF present in an F(+) strain with colicinogenic factor E(1) inhibited the transfer of the colicinogenic factor, whereas the transfer of colicinogenic factor I was not suppressed by RTF. A hypothesis that the genetic determinant of RTF controlling its "transfer system" is epistatic to that of F-factor is presented. It was also shown that F-factor present in recipients slightly suppresses the acceptance of RTF and that RTF present in recipients slightly reduces the acceptance of F-factor, F', and host chromosomes. On the other hand, the acceptance of RTF by recipients in which RTF is present was found to be about 1% of that in recipients without RTF.
渡边，T.（日本东京庆应义塾大学）和深泽，T. 肠杆菌科中附加体介导的耐药性转移。IV. 大肠杆菌K-12中耐药性转移因子与F因子之间的相互作用。《细菌学杂志》83:727 - 735。1962年。——发现F(+)和F'(+)菌株中存在的耐药性转移因子（RTF）能完全抑制F因子、F'和宿主染色体的转移。另一方面，发现Hfr菌株中存在RTF会使重组频率降低至Hfr菌株中重组频率的约10^(-2)，与Hfr的类型无关。带有产大肠杆菌素因子E(1)的F(+)菌株中存在的RTF抑制了产大肠杆菌素因子的转移，而RTF不抑制产大肠杆菌素因子I的转移。提出了一个假说，即控制其“转移系统”的RTF的遗传决定因素对F因子的遗传决定因素具有上位性。还表明受体中存在的F因子会轻微抑制RTF的接受，而受体中存在的RTF会轻微降低F因子、F'和宿主染色体的接受。另一方面，发现存在RTF的受体对RTF的接受约为不存在RTF的受体的1%。
