Forsgren A, Quie P G
Immunology. 1974 Jun;26(6):1251-6.
The influence of the alternative pathway of complement activation on opsonization of and for phagocytosis was selectively studied by chelating serum with 10 mM EGTA and 10 mM MgC1. Opsonic activity of the chelated serum depended on the alternative pathway of complement activation. The classical pathway was inhibited. There was no phagocytosis of in chelated normal serum, suggesting that activation of complement activity by the classical pathway is necessary for opsonization of this micro-organism. In control experiments when opsonization of was accomplished by IgG separated from hyperimmune serum, without the requirement of complement activity, there was no inhibition of phagocytosis by EGTA and magnesium chloride. and were opsonized in chelated normal serum, indicating that the alternative pathway of complement activation is involved in opsonization of these micro-organisms.
通过用10 mM乙二醇双四乙酸（EGTA）和10 mM氯化镁螯合血清，选择性地研究了补体激活替代途径对[具体微生物1]和[具体微生物2]吞噬调理作用的影响。螯合血清的调理活性取决于补体激活的替代途径。经典途径受到抑制。在螯合的正常血清中没有[具体微生物1]的吞噬作用，这表明经典途径激活补体活性对于该微生物的调理作用是必要的。在对照实验中，当通过从超免疫血清中分离的IgG完成[具体微生物2]的调理作用而不需要补体活性时，EGTA和氯化镁对吞噬作用没有抑制作用。[具体微生物1]和[具体微生物2]在螯合的正常血清中被调理，表明补体激活替代途径参与了这些微生物的调理作用。
