Adler J, Epstein W
Proc Natl Acad Sci U S A. 1974 Jul;71(7):2895-9. doi: 10.1073/pnas.71.7.2895.
For D-glucose and analogs there are two distinct phosphotransferase enzymes II with different specificities. Transport and chemotaxis were studied in E. coli mutants having only one or the other of these two enzymes. It was found that the transport specificity of a given enzyme II correlates with taxis specificity, and mutational loss of an enzyme II abolishes taxis toward only those sugars which it alone transports. Although enzyme I and the phosphate-carrier protein are required for full D-glucose taxis, it could not be determined if phosphorylation and transport are also required.
对于D-葡萄糖及其类似物,存在两种具有不同特异性的独特磷酸转移酶II。在仅具有这两种酶中的一种的大肠杆菌突变体中研究了运输和趋化性。发现给定的酶II的运输特异性与趋化性特异性相关,并且酶II的突变失活仅消除了对其单独运输的那些糖的趋化性。尽管完整的D-葡萄糖趋化性需要酶I和磷酸载体蛋白,但无法确定磷酸化和运输是否也是必需的。