通过蛋白水解作用从淋巴细胞表面去除的免疫球蛋白的恢复。
Recovery of immunoglobulin removed from lymphocytic surfaces by proteolysis.
作者信息
Eady R P, Hough D W, Kilshaw P J, Stevenson G T
出版信息
Immunology. 1974 Mar;26(3):549-61.
Abstract
The removal of immunoglobulin from the surfaces of human lymphocytes by limited proteolysis has been monitored by radioimmunoassay of the supernatants and immunolabelling of the residual membrane. Sublethal amounts of papain or trypsin can rapidly remove at least the immunoglobulin Fab regions from both normal and leukaemic lymphocytes. The Fabμ regions survive the high enzyme: substrate ratios prevailing, and thus are recoverable from the digests.
摘要
通过对上清液进行放射免疫测定以及对残留膜进行免疫标记，监测了通过有限蛋白酶解从人淋巴细胞表面去除免疫球蛋白的过程。亚致死量的木瓜蛋白酶或胰蛋白酶可迅速从正常淋巴细胞和白血病淋巴细胞中至少去除免疫球蛋白的Fab区域。Fabμ区域在高酶与底物比例的情况下仍能存活，因此可从消化物中回收。
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