Vitetta E S, Uhr J W
J Exp Med. 1972 Oct 1;136(4):676-96. doi: 10.1084/jem.136.4.676.
Turnover and release of cell surface Ig and secretion of total intracellular Ig has been studied in small lymphocytes from normal mouse spleen. The major findings to emerge are: (a) small lymphocytes secrete 8S IgM and IgG. A small portion of the 8S IgM, but virtually none of the IgG appears to have a cell surface phase. (b) Cell surface IgM is actively turned over with a half-life of 6-8 hr, and turnover can be accounted for by release into the incubation medium. Release is temperature dependent. (c) Released cell surface Ig is noncovalently bound to a fragment of plasma membrane. (d) H-2 antigens are not released during short-term incubation. Based on the above findings, we propose a model for the transport and release of both cell surface and conventionally secreted Ig.
对正常小鼠脾脏小淋巴细胞的细胞表面免疫球蛋白(Ig)的周转与释放以及细胞内总Ig的分泌进行了研究。主要研究结果如下:(a)小淋巴细胞分泌8S IgM和IgG。8S IgM的一小部分似乎经历细胞表面阶段,但实际上IgG几乎没有经历此阶段。(b)细胞表面IgM以6 - 8小时的半衰期进行活跃周转,周转可通过释放到培养液中来解释。释放依赖于温度。(c)释放的细胞表面Ig通过非共价键与质膜片段结合。(d)在短期培养期间,H - 2抗原不会释放。基于上述发现,我们提出了一个关于细胞表面Ig和传统分泌Ig的转运与释放的模型。