稀盐酸对酵母苯丙氨酸转运核糖核酸和大肠杆菌甲硫氨酸起始转运核糖核酸的影响。
Effects of dilute HCl on yeast tRNAPhe and E. coli tRNA1fMet.
作者信息
Ladner J E, Schweizer M P
出版信息
Nucleic Acids Res. 1974 Feb;1(2):183-92. doi: 10.1093/nar/1.2.183.
Abstract
HCl treatment of yeast tRNA(Phe) under conditions generally used for excision of ;Y' base results in structure and conformation changes as monitored by line widths in the PMR spectra at 220 MHz and by optical rotation. Like exposure of E. coli tRNA(fMet) (1) causes similar changes in the PMR spectra and optical rotation although no residues are eliminated. Electrophoresis in polyacrylamide gels provides evidence for aggregation in HCl-treated tRNA(fMet) (1). One must thus consider a general effect of HCl exposure as well as possible residue removal in assessing induced structural and conformation changes in tRNA.
摘要
在通常用于切除“Y”碱基的条件下，用盐酸处理酵母苯丙氨酸转运核糖核酸（tRNA(Phe)），会导致结构和构象发生变化，这可通过220兆赫的核磁共振谱（PMR）中的线宽以及旋光性来监测。类似地，大肠杆菌甲硫氨酸转运核糖核酸（tRNA(fMet)）暴露于相同条件下（1），尽管没有残基被去除，但也会在核磁共振谱和旋光性上引起类似的变化。在聚丙烯酰胺凝胶中进行电泳，为盐酸处理过的甲硫氨酸转运核糖核酸（tRNA(fMet)）（1）中的聚集现象提供了证据。因此，在评估转运核糖核酸中诱导的结构和构象变化时，必须考虑盐酸暴露的一般影响以及可能的残基去除情况。
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