Moss S H, Davies D J
J Bacteriol. 1974 Oct;120(1):15-23. doi: 10.1128/jb.120.1.15-23.1974.
Investigation of the effect of photoreactivation, excision, and recombination repair, individually and in combination, on the survival of ultraviolet-irradiated Escherichia coli K-12 mutants has led to a possible explanation of the loss of photoreactivability and of the complex changes in viability observed during liquid-holding. It is suggested that at higher ultraviolet light doses the excision repair mechanism becomes saturated due to overlapping of excised regions on opposite strands of the deoxyribonucleic acid helix. The results also provide support for the existing hypothesis that states that the shape of shouldered survival curves of ultraviolet-irradiated bacteria can be described in terms of the probability of occurrance of overlapping excised regions. Using the data obtained with repair-deficient mutants with closely related genetic makeup, we present a mathematical model that accurately predicts the shape of the observed survival curves and provides an estimate of the number of nucleotides in each fragment of deoxyribonucleic acid removed by the excision repair mechanism.
对光复活、切除和重组修复单独及联合作用于紫外线照射的大肠杆菌K-12突变体存活情况的研究，为光复活能力丧失以及在液体保存期间观察到的活力复杂变化提供了一种可能的解释。有人提出，在较高的紫外线剂量下，由于脱氧核糖核酸螺旋相对链上切除区域的重叠，切除修复机制会饱和。这些结果也为现有的假说提供了支持，该假说认为紫外线照射细菌的带肩存活曲线的形状可以用切除区域重叠发生的概率来描述。利用从具有密切相关基因组成的修复缺陷突变体获得的数据，我们提出了一个数学模型，该模型能准确预测观察到的存活曲线的形状，并估计切除修复机制去除的脱氧核糖核酸每个片段中的核苷酸数量。
