噬菌体φX174基因组上5-甲基胞嘧啶基团的位置。
Location of the 5-methylcytosine group on the bacteriophage phi X174 genome.
作者信息
Lee A S, Sinsheimer R L
出版信息
J Virol. 1974 Oct;14(4):872-7. doi: 10.1128/JVI.14.4.872-877.1974.
Abstract
Bacteriophage phiX174 DNA was labeled in vivo with [methyl-(3)H]methionine. The methyl-labeled progeny DNA was extracted from purified bacteriophage phiX174 particles and was used as template for in vitro synthesis of the complementary strand in the presence of the nucleoside triphosphates and Escherichia coli polymerase I. The resultant replicative form DNA was then cleaved, in separate experiments, with restriction endonucleases from Haemophilus influenzae and H. aegyptius. The DNA fragments were analyzed by polyacrylamide gel electrophoresis. It is concluded that the single methylcytosine in the viral DNA is located in a specific region of the phiX174 genome, very likely in gene H.
摘要
噬菌体φX174 DNA在体内用[甲基-(3)H]甲硫氨酸进行标记。从纯化的噬菌体φX174颗粒中提取甲基标记的子代DNA，并在核苷三磷酸和大肠杆菌聚合酶I存在的情况下用作体外合成互补链的模板。然后，在单独的实验中，用来自流感嗜血杆菌和埃及嗜血杆菌的限制性内切酶切割所得的复制型DNA。通过聚丙烯酰胺凝胶电泳分析DNA片段。得出的结论是，病毒DNA中的单个甲基胞嘧啶位于φX174基因组的特定区域，很可能在基因H中。
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