Curley D M, Adler L T, Fishman M
Immunology. 1974 Oct;27(4):553-62.
The primary response of cultured rabbit spleen cells to solubilized T2 phage (S-T2) was used to study the cellular requirements for immune in-induction. Rabbit lymph node cells, in contrast to spleen cells, were found not to respond to S-T2, and the addition of spleen cells insufficient in number to give rise to antibody in themselves failed to supply a missing cell type to lymph node cell cultures. The period in culture during which antibody formation by spleen cells could be induced was shown to be transitory, in that addition of S-T2 even 24 hours after culture initiation resulted in little, if any, antibody production. Cultures of non-adherent cells from spleen, obtained by removal of adherent cells on plastic dishes or glass bead columns, by silica treatment for removal of phagocytic cells, or by sequential treatment by adherence and with silica, responded as well to S-T2 as did the whole spleen cell population. These findings strongly indicate the relative macrophage independence of the response to S-T2 and are suggestive of the role of macrophages in solubilization of antigens, since intact phage fails to induce antibody formation in macrophage-deprived cultures.
利用培养的兔脾细胞对可溶性T2噬菌体（S-T2）的初次反应来研究免疫诱导的细胞需求。与脾细胞不同，兔淋巴结细胞对S-T2无反应，而且向数量不足以自身产生抗体的脾细胞中添加脾细胞，也无法为淋巴结细胞培养物提供缺失的细胞类型。结果表明，脾细胞产生抗体的培养期是短暂的，因为即使在培养开始24小时后添加S-T2，产生的抗体也极少（如果有的话）。通过在塑料培养皿或玻璃珠柱上去除贴壁细胞、用二氧化硅处理以去除吞噬细胞或通过贴壁和二氧化硅顺序处理获得的脾非贴壁细胞培养物，对S-T2的反应与整个脾细胞群体一样好。这些发现有力地表明了对S-T2的反应相对不依赖巨噬细胞，并且提示了巨噬细胞在抗原溶解中的作用，因为完整的噬菌体在缺乏巨噬细胞的培养物中不能诱导抗体形成。
