Feldmann M, Palmer J
Immunology. 1971 Oct;21(4):685-99.
Tissue culture techniques were combined with cell separation procedures, antimacrophage serum and soluble and particulate forms of sheep red cell antigen to investigate the cellular requirements for a secondary antibody response. By using the highly efficient active adherence column separation method of Shortman which almost completely removes phagocytic cells, it was found that the secondary response to SRC was macrophage dependent. This conclusion was verified by the use of specific antimacrophage serum and by a combination of both methods. Critical tests were used to verify that these separation methods acted on phagocytic and not on other cells, for example, thymus or bone marrow derived lymphocytes. In contrast, the secondary immune response to POL and solubilized SRC antigen was not dependent on the presence of phagocytic cells, as highly purified lymphocytes responded normally to these antigens. Antimacrophage serum did not depress the immune response to these soluble antigens. These results indicate that the requirement for macrophage activity depends on the physical size of the antigen. These findings, obtained in the secondary response , were closely analogous to previous findings from this laboratory on the cellular basis of the primary response to SRC and POL. The reasons for the different cellular bases of the secondary immune response to various molecular forms of antigen are discussed. The similar cellular basis of the primary and secondary response suggests that the basic mechanisms of immunization in these responses are the same.
组织培养技术与细胞分离程序、抗巨噬细胞血清以及绵羊红细胞抗原的可溶性和颗粒性形式相结合，以研究二次抗体应答的细胞需求。通过使用肖特曼的高效活性黏附柱分离方法，该方法几乎能完全去除吞噬细胞，发现对绵羊红细胞（SRC）的二次应答依赖于巨噬细胞。使用特异性抗巨噬细胞血清以及两种方法相结合，验证了这一结论。采用关键试验来验证这些分离方法作用于吞噬细胞而非其他细胞，例如胸腺或骨髓来源的淋巴细胞。相比之下，对多聚赖氨酸（POL）和可溶性SRC抗原的二次免疫应答不依赖于吞噬细胞的存在，因为高度纯化的淋巴细胞对这些抗原能正常应答。抗巨噬细胞血清并未抑制对这些可溶性抗原的免疫应答。这些结果表明对巨噬细胞活性的需求取决于抗原的物理大小。在二次应答中获得的这些发现与本实验室先前关于对SRC和POL初次应答的细胞基础的发现极为相似。讨论了针对不同分子形式抗原的二次免疫应答具有不同细胞基础的原因。初次和二次应答相似的细胞基础表明这些应答中免疫的基本机制是相同的。
