携带鼠伤寒沙门氏菌组氨酸操纵子突变的φ80 dhis构建体。
Construction of phi80 dhis carrying Salmonella typhimurium histidine operon mutations.
作者信息
Smith G R, Tong B
出版信息
J Bacteriol. 1974 Dec;120(3):1223-6. doi: 10.1128/jb.120.3.1223-1226.1974.
Abstract
Starting with a previously isolated F'his episome and phi80 dhis imm(lambda) cI857 transducing phage, we have constructed recombinant elements bearing previously isolated his mutations from Salmonella typhimurium. These phages were constructed as sources of deoxyribonucleic acid for in vitro biochemical experiments on gene regulation. The manipulation of genes between S. typhimurium and Escherichia coli described here may be useful in studying other S. typhimurium operons.
摘要
从先前分离的F'his附加体和phi80 dhis imm(lambda) cI857转导噬菌体开始，我们构建了带有先前从鼠伤寒沙门氏菌分离的his突变的重组元件。构建这些噬菌体作为用于基因调控体外生化实验的脱氧核糖核酸来源。这里描述的鼠伤寒沙门氏菌和大肠杆菌之间的基因操作可能有助于研究其他鼠伤寒沙门氏菌操纵子。
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