Avitabile A, Carlomagno-Cerillo S, Favvre R, Blasi F
J Bacteriol. 1972 Oct;112(1):40-7. doi: 10.1128/jb.112.1.40-47.1972.
In vitro studies have been of great value in elucidating the mechanism of the regulation of several bacterial operons. To obtain a deoxyribonucleic acid preparation enriched for the histidine (his) and for the isoleucine-valine (ilv) operons, we have isolated bacteriophages carrying the his and the ilv regions of the Escherichia coli chromosome. Transposition of the his operon to a site close to the att80 region of the E. coli chromosome has been carried out selecting for integration of a temperature-sensitive F'his(+) in the tonB locus. This transposed strain has been lysogenized with phi80i(lambda). Upon induction of the lysogen, His(+) transductants have been isolated, which, on further induction give rise to HFT (high frequency of transduction) lysates. Preliminary characterization of the transducing phage is reported. The ilv operon, carried on an F' particle, has been fused to an episome carrying the att80 region. The fused episome has been lysogenized with phi80i lambdat68. Upon induction of the lysogen, Ilv(+) transductants have been isolated which on further induction give rise to HFT lysates.
体外研究在阐明几种细菌操纵子的调控机制方面具有重要价值。为了获得富含组氨酸（his）和异亮氨酸 - 缬氨酸（ilv）操纵子的脱氧核糖核酸制剂，我们分离了携带大肠杆菌染色体his和ilv区域的噬菌体。已将his操纵子转座到靠近大肠杆菌染色体att80区域的位点，通过选择温度敏感型F'his(+)整合到tonB位点来实现。该转座菌株已用phi80i(λ)进行溶源化。诱导溶源菌后，分离出His(+)转导子，进一步诱导可产生高频转导（HFT）裂解物。报告了转导噬菌体的初步特征。携带在F'颗粒上的ilv操纵子已与携带att80区域的附加体融合。融合的附加体已用phi80iλt68进行溶源化。诱导溶源菌后，分离出Ilv(+)转导子，进一步诱导可产生HFT裂解物。
