不稳定抑制剂对酶的不可逆抑制动力学
Kinetics of irreversible enzyme inhibition by an unstable inhibitor.
作者信息
Rakitzis E T
出版信息
Biochem J. 1974 Aug;141(2):601-3. doi: 10.1042/bj1410601.
Abstract
A mathematical treatment for the general case of enzyme inactivation by an inhibitor that breaks down in solution in a first-order reaction is presented. Cathepsin D was inactivated by fluorescein isothiocyanate with a K(i) of 4.47mum. Kinetic constants were also determined for the inactivation of cathepsin D by 1,1-bis(diazoacetyl)-2-phenylethane, and the inactivation of pepsin C by diazoacetyl-dl-norleucine methyl ester.
摘要
本文给出了一种抑制剂在溶液中按一级反应分解时酶失活一般情况的数学处理方法。组织蛋白酶D被异硫氰酸荧光素失活，其抑制常数（K(i)）为4.47μM。还测定了1,1 - 双（重氮乙酰基）- 2 - 苯乙烷使组织蛋白酶D失活以及重氮乙酰 - dl - 正亮氨酸甲酯使胃蛋白酶C失活的动力学常数。
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Kinetics of irreversible enzyme inhibition by an unstable inhibitor.不稳定抑制剂对酶的不可逆抑制动力学
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本文引用的文献
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Some properties of specific cholinesterase with particular reference to the mechanism of inhibition by diethyl p-nitrophenyl thiophosphate (E 605) and analogues.特异性胆碱酯酶的某些特性，特别涉及对硫代磷酸O,O-二乙基对硝基苯酯（E605）及其类似物的抑制机制。
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The kinetics of the reaction of N,N-dimethyl-2-phenylaziridinium ion with bovine erythrocyte acetylcholinesterase.N,N-二甲基-2-苯基氮杂环丙烷离子与牛红细胞乙酰胆碱酯酶反应的动力学
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Experimental evidence for a hydrophobic active center of glutamic-aspartic transaminase. Specific interaction of holoenzyme and apoenzyme with two fluorescein derivatives.谷氨酸-天冬氨酸转氨酶疏水活性中心的实验证据。全酶和脱辅酶与两种荧光素衍生物的特异性相互作用。
Eur J Biochem. 1973 Oct 18;38(3):537-52. doi: 10.1111/j.1432-1033.1973.tb03089.x.
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Preparation and properties of lysozyme modified by fluorescein-isothiocyanate.异硫氰酸荧光素修饰溶菌酶的制备及性质
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The inhibition of cholinesterase by diethyl phosphorochloridate.二乙基氯磷酸酯对胆碱酯酶的抑制作用。
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