大肠杆菌K-12中异亮氨酸和缬氨酸的代谢：ilv特异性信使核糖核酸的检测与测定
Isoleucine and valine metabolism in Escherichia coli K-12: detection and measurement of ilv-specific messenger ribonucleic acid.
作者信息
Vonder Haar R A, Umbarger H E
出版信息
J Bacteriol. 1974 Nov;120(2):687-96. doi: 10.1128/jb.120.2.687-696.1974.
Abstract
Ribonucleic acid-deoxyribonucleic acid (RNA-DNA) hybridization was employed for the determination of messenger RNA transcribed from the ilv gene cluster of Escherichia coli K-12. Strains with derepressed levels of the isoleucine and valine biosynthetic enzymes owing to linked or unlinked genetic lesions were found to exhibit ilv messenger RNA levels from 1.5- to 4-fold higher than did their isogenic parents. When grown under conditions that specifically repressed the synthesis of isoleucine- and valine-forming enzymes, most strains exhibited drastically reduced ilv messenger RNA levels. Hybridization performed with the separated strands of ilv DNA showed that all the ilv genes are transcribed from the same strand, the "l strand" of lambdaphi80CI857St68dilv DNA. Sucrose gradient analyses of RNA extracted from cells starved for isoleucine, valine, or leucine resulted in the detection of at least two distinct types of ilv messenger RNA.
摘要
采用核糖核酸 - 脱氧核糖核酸（RNA - DNA）杂交技术来测定从大肠杆菌K - 12的ilv基因簇转录的信使RNA。由于连锁或非连锁遗传损伤而导致异亮氨酸和缬氨酸生物合成酶水平去阻遏的菌株，其ilv信使RNA水平比同基因亲本高1.5至4倍。当在特异性抑制异亮氨酸和缬氨酸形成酶合成的条件下生长时，大多数菌株的ilv信使RNA水平大幅降低。用ilv DNA的单链进行杂交表明，所有ilv基因都从同一链转录，即λphi80CI857St68dilv DNA的“l链”。对缺乏异亮氨酸、缬氨酸或亮氨酸的饥饿细胞中提取的RNA进行蔗糖梯度分析，结果检测到至少两种不同类型的ilv信使RNA。
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