Thompson E D, Parks L W
J Bacteriol. 1974 Nov;120(2):779-84. doi: 10.1128/jb.120.2.779-784.1974.
The function of sterols in mitochondrial structures of yeast was examined. Sterol mutant strains were employed to examine the effects of altered sterolic content on optimal and permissive growth temperatures in respiring and fermenting cultures. Although fermentative growth was unaffected by sterol composition, a definite decrease in both the optimal and the permissive growth temperatures of respiring cultures was observed when ergosterol was replaced by Delta(8(9), 22)-ergostadiene-3beta-ol. In vitro studies showed a similar decrease in membrane phase transition temperatures of the mitochondrial enzyme S-adenosylmethionine: Delta(24)-sterol methyltransferase in the mutant strains. Increased sterol and methyltransferase levels were detected in strains incapable of synthesizing ergosterol. A possible control function governing sterol synthesis is proposed for ergosterol.
研究了酵母线粒体结构中甾醇的功能。使用甾醇突变菌株来检测甾醇含量改变对呼吸和发酵培养物中最佳生长温度和允许生长温度的影响。尽管发酵生长不受甾醇组成的影响，但当麦角甾醇被Δ(8(9),22)-麦角甾二烯-3β-醇取代时，观察到呼吸培养物的最佳生长温度和允许生长温度均明显降低。体外研究表明，突变菌株中线粒体酶S-腺苷甲硫氨酸：Δ(24)-甾醇甲基转移酶的膜相变温度也有类似降低。在无法合成麦角甾醇的菌株中检测到甾醇和甲基转移酶水平升高。提出了麦角甾醇对甾醇合成可能的控制作用。
