酿酒酵母中甾醇C-14去甲基化缺陷的生存能力对第二种甾醇生物合成突变的需求。
Requirement for a second sterol biosynthetic mutation for viability of a sterol C-14 demethylation defect in Saccharomyces cerevisiae.
作者信息
Taylor F R, Rodriguez R J, Parks L W
出版信息
J Bacteriol. 1983 Jul;155(1):64-8. doi: 10.1128/jb.155.1.64-68.1983.
Abstract
Genetic analysis of a nystatin-resistant sterol mutant (strain JR4) of Saccharomyces cerevisiae defective in C-14 demethylation revealed the presence of a second mutation in 5,6-desaturation. It appeared from complementation tests that a defect in delta 5-desaturase enzyme activity was required for the viability of the C-14 demethylation mutant. Growth studies with a sterol auxotrophic strain indicated that the major sterol of strain JR4, 14 alpha-methyl-ergosta-8,24(28)-dien-3 beta-ol, could satisfy "bulk" membrane requirements but not the second, structurally specific, sterol function that we defined previously (Rodriguez et al., Biochem. Biophys. Res. Commun. 106:435-441, 1982). Leakiness in the sterol mutations in strain JR4 provided a small amount of ergosterol which could satisfy this second function.
摘要
对酿酒酵母中一种在C-14去甲基化方面存在缺陷的制霉菌素抗性固醇突变体（菌株JR4）进行的遗传分析显示，在5,6-去饱和作用中存在第二个突变。从互补试验来看，C-14去甲基化突变体的存活似乎需要δ5-去饱和酶活性存在缺陷。对一种固醇营养缺陷型菌株的生长研究表明，菌株JR4的主要固醇，即14α-甲基-麦角甾-8,24(28)-二烯-3β-醇，能够满足“大量”膜的需求，但不能满足我们之前定义的第二种结构特异性固醇功能（Rodriguez等人，《生物化学与生物物理学研究通讯》106:435 - 441, 1982）。菌株JR4中固醇突变的渗漏产生了少量的麦角固醇，其可以满足这第二种功能。
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