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Requirement for a second sterol biosynthetic mutation for viability of a sterol C-14 demethylation defect in Saccharomyces cerevisiae.酿酒酵母中甾醇C-14去甲基化缺陷的生存能力对第二种甾醇生物合成突变的需求。
J Bacteriol. 1983 Jul;155(1):64-8. doi: 10.1128/jb.155.1.64-68.1983.
2
Differences in the cytochrome P-450 enzymes of sterol C-14 demethylase mutants of Saccharomyces cerevisiae.酿酒酵母固醇C-14脱甲基酶突变体中细胞色素P-450酶的差异。
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Sterol synthesis and viability of erg11 (cytochrome P450 lanosterol demethylase) mutations in Saccharomyces cerevisiae and Candida albicans.酿酒酵母和白色念珠菌中erg11（细胞色素P450羊毛甾醇去甲基酶）突变体的甾醇合成与生存能力
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Characterization of a Saccharomyces cerevisiae mutant, N22, defective in ergosterol synthesis and preparation of [28-14C]ergosta-5,7-dien-3 beta-ol with the mutant.酿酒酵母突变体N22的特性研究，该突变体在麦角固醇合成方面存在缺陷，以及利用该突变体制备[28-¹⁴C]麦角甾-5,7-二烯-3β-醇。
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Rate-limiting steps in the Saccharomyces cerevisiae ergosterol pathway: towards improved ergosta-5,7-dien-3β-ol accumulation by metabolic engineering.酿酒酵母麦角固醇途径中的限速步骤：通过代谢工程提高麦角甾-5,7-二烯-3β-醇的积累。
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Biochem Biophys Res Commun. 1989 Nov 15;164(3):1170-5. doi: 10.1016/0006-291x(89)91792-0.
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Deletion of the Candida glabrata ERG3 and ERG11 genes: effect on cell viability, cell growth, sterol composition, and antifungal susceptibility.光滑念珠菌ERG3和ERG11基因缺失：对细胞活力、细胞生长、甾醇组成及抗真菌药敏性的影响
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Deletion of the Candida glabrata ERG3 and ERG11 genes: effect on cell viability, cell growth, sterol composition, and antifungal susceptibility.光滑念珠菌ERG3和ERG11基因缺失：对细胞活力、细胞生长、甾醇组成及抗真菌药敏性的影响
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Cross-resistance to polyene and azole drugs in Cryptococcus neoformans.新型隐球菌对多烯类和唑类药物的交叉耐药性。
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本文引用的文献

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Effect of alkyl-substituted precursors of cholesterol on artificial and natural membranes and on the viability of Mycoplasma capricolum.胆固醇烷基取代前体对人工和天然膜以及山羊支原体生存能力的影响。
Biochemistry. 1980 Apr 1;19(7):1462-7. doi: 10.1021/bi00548a031.
2
Effects of cycloartenol and lanosterol on artificial and natural membranes.环阿屯醇和羊毛甾醇对人工膜和天然膜的影响。
Biochem Biophys Res Commun. 1980 Jan 15;92(1):221-8. doi: 10.1016/0006-291x(80)91542-9.
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Application of high-performance liquid chromatographic separation of free sterols to the screening of yeast sterol mutants.高效液相色谱法分离游离甾醇在酵母甾醇突变体筛选中的应用
Anal Biochem. 1982 Jan 1;119(1):200-4. doi: 10.1016/0003-2697(82)90686-8.
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An assessment of the specificity of sterol uptake and esterification in Saccharomyces cerevisiae.酿酒酵母中固醇摄取和酯化特异性的评估。
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Utilization and metabolism of methyl-sterol derivatives in the yeast mutant strain GL7.酵母突变株GL7中甲基甾醇衍生物的利用与代谢
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Corresponding changes in kynurenine hydroxylase activity, membrane fluidity, and sterol composition in Saccharomyces cerevisiae mitochondria.酿酒酵母线粒体中犬尿氨酸羟化酶活性、膜流动性和甾醇组成的相应变化。
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Adaptation of Saccharomyces cerevisiae to growth on cholesterol: selection of mutants defective in the formation of lanosterol.酿酒酵母对胆固醇生长环境的适应性：筛选羊毛甾醇合成缺陷型突变体。
Biochem Biophys Res Commun. 1980 Aug 29;95(4):1437-45. doi: 10.1016/s0006-291x(80)80058-1.
8
A requirement for ergosterol to permit growth of yeast sterol auxotrophs on cholestanol.酵母甾醇营养缺陷型在胆甾烷醇上生长需要麦角甾醇。
Biochem Biophys Res Commun. 1982 May 31;106(2):435-41. doi: 10.1016/0006-291x(82)91129-9.
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Sterol structure and membrane function.
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Effect of altered sterol composition on growth characteristics of Saccharomyces cerevisiae.甾醇组成改变对酿酒酵母生长特性的影响。
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酿酒酵母中甾醇C-14去甲基化缺陷的生存能力对第二种甾醇生物合成突变的需求。

Requirement for a second sterol biosynthetic mutation for viability of a sterol C-14 demethylation defect in Saccharomyces cerevisiae.

作者信息

Taylor F R, Rodriguez R J, Parks L W

出版信息

J Bacteriol. 1983 Jul;155(1):64-8. doi: 10.1128/jb.155.1.64-68.1983.

DOI:10.1128/jb.155.1.64-68.1983
PMID:6345514
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC217652/
Abstract

Genetic analysis of a nystatin-resistant sterol mutant (strain JR4) of Saccharomyces cerevisiae defective in C-14 demethylation revealed the presence of a second mutation in 5,6-desaturation. It appeared from complementation tests that a defect in delta 5-desaturase enzyme activity was required for the viability of the C-14 demethylation mutant. Growth studies with a sterol auxotrophic strain indicated that the major sterol of strain JR4, 14 alpha-methyl-ergosta-8,24(28)-dien-3 beta-ol, could satisfy "bulk" membrane requirements but not the second, structurally specific, sterol function that we defined previously (Rodriguez et al., Biochem. Biophys. Res. Commun. 106:435-441, 1982). Leakiness in the sterol mutations in strain JR4 provided a small amount of ergosterol which could satisfy this second function.

摘要

对酿酒酵母中一种在C-14去甲基化方面存在缺陷的制霉菌素抗性固醇突变体（菌株JR4）进行的遗传分析显示，在5,6-去饱和作用中存在第二个突变。从互补试验来看，C-14去甲基化突变体的存活似乎需要δ5-去饱和酶活性存在缺陷。对一种固醇营养缺陷型菌株的生长研究表明，菌株JR4的主要固醇，即14α-甲基-麦角甾-8,24(28)-二烯-3β-醇，能够满足“大量”膜的需求，但不能满足我们之前定义的第二种结构特异性固醇功能（Rodriguez等人，《生物化学与生物物理学研究通讯》106:435 - 441, 1982）。菌株JR4中固醇突变的渗漏产生了少量的麦角固醇，其可以满足这第二种功能。