Chilina A R, Chang M, Ives D H, Koestner A
Res Commun Chem Pathol Pharmacol. 1979 May;24(2):289-305.
Differences in nuclear composition and variations in the types of acid soluble nuclear proteins were identified when cultivated neoplastic and nontransformed rat brain cells were compared. HeLa S-3 cells were used as a biological reference in these studies. The nuclei of anaplastic glioma cells were found to contain more total nuclear protein and greater amounts of nuclear RNA, than the nuclei of nontransformed embryonic and neonatal rat brain cells. Densitometer profiles of samples developed by polyacrylamide gel electrophoresis indicated that all three major histone classes were present in the nuclei of the four different cell types examined. Each type of cell was grown in the presence of 3H-lysine or 3H-lysine plus 14C-arginine to further characterize both the histones and the acid soluble nonhistone proteins. Neoplastic and nontransformed cells contained similar quantities of histones H4, H3, H2A and H2B. In contrast, transformed cells were found to contain two dominant subspecies of lysine rich H1 histone, while only one histone H1 subcomponent was extracted from the nuclei of the embryonic and neonatal rat brain cells. Nuclei isolated from HeLa cells and anaplastic glioma cells also contained increased varieties and larger quantities of acid soluble nonhistone nuclear proteins.
当比较培养的肿瘤性和未转化的大鼠脑细胞时，发现了核组成的差异以及酸溶性核蛋白类型的变化。在这些研究中，使用HeLa S-3细胞作为生物学对照。发现间变性胶质瘤细胞的细胞核比未转化的胚胎和新生大鼠脑细胞的细胞核含有更多的总核蛋白和大量的核RNA。聚丙烯酰胺凝胶电泳显示的样品光密度计图谱表明，在所检测的四种不同细胞类型的细胞核中均存在所有三种主要组蛋白。每种细胞类型都在3H-赖氨酸或3H-赖氨酸加14C-精氨酸的存在下生长，以进一步表征组蛋白和酸溶性非组蛋白。肿瘤性和未转化的细胞含有相似数量的组蛋白H4、H3、H2A和H2B。相比之下，发现转化细胞含有两种主要的富含赖氨酸的H1组蛋白亚种，而从胚胎和新生大鼠脑细胞的细胞核中仅提取到一种组蛋白H1亚组分。从HeLa细胞和间变性胶质瘤细胞中分离出的细胞核还含有更多种类和更大数量的酸溶性非组蛋白核蛋白。
