用罗曼诺夫斯基染料对人类带型染色体进行染色：染色性质的一些实际影响。

Staining banded human chromosomes with Romanovsky dyes: some practical consequences of the nature of the stain.

作者信息

Curtis D J, Horobin R W

出版信息

Humangenetik. 1975;26(2):99-104. doi: 10.1007/BF00278435.

Abstract

Chromatographic analyses of commercial samples of Romanovsky stains were followed by various chemical and staining studies. Strong banding of chromosomes was found to be favoured by the presence in the stain of high concentrations of methylene violet Bernthsen and its immediate homologues. Chromatographic analysis showed that these dyes are present in dry Leishman and Giemsa powders only in small proportion. However, additional active dye may be generated during the process of making up staining solutions from the dry powders, if the methanol used is not contaminated with formic acid and if the period of heating is not too brief.

摘要

对罗曼诺夫斯基染色剂的商业样品进行色谱分析后，进行了各种化学和染色研究。结果发现，高浓度的亚甲蓝伯恩森及其紧邻同系物存在于染色剂中时，有利于染色体出现强烈的条带。色谱分析表明，这些染料在干燥的利什曼和吉姆萨粉末中仅占小部分。然而，如果所用甲醇未被甲酸污染且加热时间不过短，则在由干粉配制染色溶液的过程中可能会产生额外的活性染料。