Lerner H R, Ben-Bassat D, Reinhold L, Poljakoff-Mayber A
Department of Botany, The Hebrew University, Jerusalem, Israel.
Plant Physiol. 1978 Feb;61(2):213-7. doi: 10.1104/pp.61.2.213.
Treatment with aqueous toluene-ethanol has been shown to induce "pore" formation in plant cell membranes. The evidence is as follows: [List: see text]While the principal experimental material was roots of Atriplex nummularia Lindl., the fact that similar results were also observed with leaves of Pisum sativum L. and with the alga Chlorella pyrenoidosa Chik. suggests that the phenomenon is general.Although the phenomenon of pore induction is qualitatively similar to that in microorganisms, the pores induced appear to be smaller. It is proposed that induced leakage could be the basis for the development of simple and rapid methods for plant biochemical studies.
已证明用甲苯 - 乙醇水溶液处理可诱导植物细胞膜形成“孔”。证据如下:[列表:见正文]虽然主要实验材料是滨藜(Atriplex nummularia Lindl.)的根,但在豌豆(Pisum sativum L.)叶片和小球藻(Chlorella pyrenoidosa Chik.)中也观察到类似结果,这表明该现象具有普遍性。尽管诱导孔形成的现象在性质上与微生物中的相似,但诱导产生的孔似乎更小。有人提出,诱导渗漏可能是开发用于植物生化研究的简单快速方法的基础。