真菌菌丝体中游离氨基酸库的分析。
Analysis of free amino acid pools in fungal mycelia.
作者信息
Heathcote J G, Davies D M, Haworth C
出版信息
Appl Microbiol. 1972 Feb;23(2):349-53. doi: 10.1128/am.23.2.349-353.1972.
Abstract
Free amino acid pools derived from three different types of fungal mycelia have been analyzed by the method of Heathcote and Haworth by using thinlayer chromatography. The preliminary extraction was carried out with boiling water and interfering proteins; peptides and salts were first removed by means of an ion-retardation resin. As far as determined, the results obtained represent the first quantitative analysis of fungal amino acid pools.
摘要
通过希思科特和霍沃思的方法，利用薄层色谱法分析了源自三种不同类型真菌菌丝体的游离氨基酸库。初步提取是用沸水进行的，干扰性蛋白质、肽和盐首先通过离子阻滞树脂去除。就已确定的情况而言，所获得的结果代表了对真菌氨基酸库的首次定量分析。
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