菠萝蛋白酶在使用自动分析仪进行抗-D定量分析中的不良影响。
The adverse effect of bromelin in anti-D quantitation using the AutoAnalyzer.
作者信息
Gunson H H, Phillips P K, Stratton F
出版信息
J Clin Pathol. 1972 Mar;25(3):206-9. doi: 10.1136/jcp.25.3.206.
Abstract
With eight of 32 sera significantly higher estimations of anti-D concentration were obtained when test cells premodified with bromelin were used instead of bromelin as a reagent added to the reaction manifold. With one serum the difference in estimation, using the two techniques, revealed a ten-fold discrepancy. The consequences of these findings are discussed in relation to the use of the AutoAnalyzer for the quantitation of anti-D.
摘要
当使用经菠萝蛋白酶预先处理的检测细胞，而非将菠萝蛋白酶作为试剂添加到反应体系中时，在32份血清中有8份血清的抗-D浓度估计值显著更高。对于一份血清，使用这两种技术得出的估计值差异显示出10倍的偏差。结合使用自动分析仪对抗-D进行定量分析，对这些发现的结果进行了讨论。
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